VARIATION IN ANTIOXIDANT POTENTIAL OF CURCUMA LONGA L. COLLECTED FROM DIFFERENT ECOLOGICAL NICHES OF WESTERN HIMALAYAN REGION

Authors

  • Neelam Arya G. B. Pant University of Agriculture & Technology, Pantnagar, Uttarakhand
  • Om Prakash G. B. Pant University of Agriculture & Technology, Pantnagar, Uttarakhand
  • A. K. Verma G. B. Pant University of Agriculture & Technology, Pantnagar, Uttarakhand
  • Vivekanand . G.B.Pant University of Agriculture and Technology, Pantnagar
  • A. K. Pant G. B. Pant University of Agriculture & Technology, Pantnagar, Uttarakhand

Keywords:

Free radical scavenging, Total antioxidant capacity, Total phenolic, Total flavonoid, Total ascorbic acid

Abstract

Objective: The present study aims to evaluate the nutritional content and antioxidant activities of twenty accessions of turmeric rhizome from various locations of Uttarakhand.

Methods: The antioxidant activity was evaluated by different method viz; DPPH radical scavenging activity, ferric reducing antioxidant power assay (FRAP), Hydroxyl radical scavenging activity, Nitric Oxide (NO) scavenging activity, Superoxide anion radical scavenging activity and nutritional value viz; total phenolic content, total flavonoid content, total ortho-dihydroxy phenols, total ascorbic acid content and total antioxidant capacity.

Results: Alcoholic extracts of different turmeric rhizomes showed wide variation in abilities to scavenge nitric oxide, hydroxyl, superoxide free radical, DPPH with IC50 values in the range of 39.03-76.14, 39.77-72.85, 43.77-77.13, 64.38-271.95 µg/ml and FRAP value 19.54-54.84 µmole. The total phenolic content, total flavonoid content, total ortho-dihydroxy phenols, total ascorbic acid content and total antioxidant capacity of different alcoholic extracts of rhizomes were found in the range of 11.19-51.49, 6.35-30.95, 0.93-8.12, 0.14-1.29 and 27.44-94.78 mg/g respectively.

Conclusion: The alcoholic extract of different accessions of Curcuma longa L. has shown excellent biochemical and antioxidant potential and therefore refers as an important natural source of antioxidant.

 

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Author Biography

Vivekanand ., G.B.Pant University of Agriculture and Technology, Pantnagar

Professor Chemistry G.B.Pant University of Agriculture and Technology, Pantnagar

References

Cai YZ, Sun M, Corke H. Antioxidant activity of betaline from plants of the Amaranthacea. J Agric Food Chem 2003;51:2288-94.

Lim YY, Murtijaya J. Antioxidant properties of Phyllanthus amarus as affected by different drying methods. lebensmittle wissenschaft. Technol 2007;40:1664-9.

Keawpradub N, Zaeoung S. Cytotoxic and free radical scavenging activities of Zingiberaceous rhizomes. Songklanakarin J Sci Technol 2005;27:799-812.

Stoilova I, Krastanov A, Stoyanova A, Denev P, Gargova S. Antioxidant activity of ginger extract. Food Chem 2007;102:764-70.

Ravindran PN. Turmeric the golden spice of life. Turmeric the genus Curcuma. Taylor Francies 2006;14(1):1-14.

Aggarwal BB, Sundaram C, Malani N, Ichikawa H. Curcumin; the Indian solid golid. Adv Exp Med BIol 2007;595:1-75.

Tang W, Eisenbrand G. Chinese drugs of plant origin. Springer Verlag, Berlin, Heidelberg. Germany 1992;613-9.

Lee H-S, Shin WK, Song C, Cho KY, Ahn YJ. Insecticidal activities of ar-turmerone identified Curcuma longa L. rhizome against Nilaparvata lugens (Homoptera: Delphacidae) and Plutella xyostella (Lepiodoptera: Yponomeutidae). J Asia-Pac Entomol 2001;4(2):181-5.

Wang Y, Lu Z, Wu H, Lv F. Study on the antibiotic activity of microcapsule curcumin aganist foodborn pathogens. Int J Food Microbiol 2009;136:71-4.

Kuttan R, Bhanumathly P, Nirmala K, George MC. Potential anticancer activity of turmeric (Curcuma longa). Cancer Lett 1985;29(2):197-202.

Wang X, Jiang Y, Wang YW, Huang MT, Ho CT, Hauang Q. Enhancing anti-inflammation activity of curcumin through O/W nanoemulsions. Food Chem 2008;108:419-24.

Ammon HPI, Wahl MA. Pharmacology of Curcuma longa L. Plant Med 1991;57:1-7.

Kiso Y, Suzuki H, Watanabe N, Oshima Y, Hikino O. Antihepatotoxic principles of Curcuma longa rhizomes. Plant Med 1983;49:185-7.

Devasagayam TPA, Sainis KB. Immune system and antioxidants especially those derive from Indian medicinal plants. Indian J Exp Biol 2002;40:639-55.

Jayaprakasha GK, Rao LJ, Sakariah KK. Antioxidant activities of curcumin, demethoxycurcumin and bisdemethoxycurcumin. Food Chem 2006;98:720-4.

Maheshwari RK, Shing AK, Gaddipati J, Srimal RC. Multiple biological activities of curcumin; A short review. Life Sci 2006;78:2081-7.

Singlenton UL, Rossi JA. Colorimetry of total phenolics with phosphomolibdic phosphotungstic acid reagent. Am J Enol Vitic 1965;16:144-58.

Choi Y, Jeong H, Lee J. Antioxidant activity of methanolic extracts from some grains consumed in Korea. Food Chem 2007;130:130-8.

Arnow LE. Colorimetric determination of the components of 3,4-dihydroxyphenylalanin-tyrosine mixtures. J Biochem 1937;118:531-7.

Prieto P, Pineda M, Aguilar M. Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: specific application to the determination of vitamin E. Anal Biochem 1999;269:337-41.

Reo JH, Kuether CA. Determination of ascorbic acid in whole blood and urine through the 2,4-dinitrophenylhydrazine derivate of ascorbic acid. J Biochem 1943;143:399-406.

Benzie IFF, Strain JJ. The ferric reducing ability of plasma as a measure of antioxidant power; the FRAP assay. Anal Biochem 1996;239:70-6.

Braca A, Sortino C, Politi M. Antioxidant activity of flavonoids from Licania licaniaeflora. J Ethnopharmacol 2002;79:379-81.

Halliwell B, Gutteridge JMC, Aruoma OI. The deoxyribose method; a simple test-tube†assay for determination of rate constants for reaction of hydroxyl radicals. Anal Biochem 1987;165:216-9.

Green LC, Wagner DA, Glogowski J, Skipper PL, Wishnok JK, Tannenbaum SR. Analysis of nitrate, nitrite and 15N in biological fluids. Anal Biochem 1982;126:131–6.

Marcocci L, Maguire JJ, Droy-Lefaix MT, Packer L. The nitric oxide scavenging property of Ginkgo biloba extract Egb 761. Biochem Biophys Res Commun 1994;201:748-55.

Nishimiki M, Rao NA, Yagi K. The occurrence of superoxide anion in the reaction of reduced phenazine methosulphate and molecular oxygen. Biochem Biophys Res Commun 1972;46:849-53.

Bryant JP, Chapin FSI, Klein DR. Carbon/nutrient balance of boreal plants in relation to vertebrate herbivory. Oikos 1983;40:357-68.

Dixon RA, Paiva NL. Stress-induced phenyepropanoid metabolism. Plant Cell 1995;7(7):1085-97.

Zhang W, Seki M, Furusaki S. Effect of temperature and its shift on growth and anthocyanin production in suspension cultures of strawberry cells. Plant Sci 1997;127:207-14.

Chalker-Scott L, Fnchigami LH. The role of phenolic compounds in plant stress responses; low temperature stress physiology in crops (paul HL. Ed. CRC press: Inc, Boca Raton, Florida Entomologist; 1989. p. 40.

Hotelling H. Analysis of a complex of statistical variables into principal components. J Educational Psychology 1933;24:417-41.

Lohani M, Singh D, Singh JP. Genetic diversity assessment through principal component analysis in potato. Veg Sci 2012;39(2):207-9.

Published

01-07-2015

How to Cite

Arya, N., O. Prakash, A. K. Verma, V. ., and A. K. Pant. “VARIATION IN ANTIOXIDANT POTENTIAL OF CURCUMA LONGA L. COLLECTED FROM DIFFERENT ECOLOGICAL NICHES OF WESTERN HIMALAYAN REGION”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 7, no. 7, July 2015, pp. 85-90, https://www.innovareacademics.in/journals/index.php/ijpps/article/view/5080.

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Original Article(s)