IN VITRO EVALUATION OF ANTIFUNGAL POTENTIAL AND ELECTRON MICROSCOPIC STUDIES OF BACILLUS AMYLOLIQUEFACIENS AGAINST ASPERGILLUS SPECIES

Authors

  • Anu Singh Centre for Biotechnology, Maharshi Dayanand University, Rohtak, Haryana, India
  • Meenakshi Balhara Centre for Biotechnology, Maharshi Dayanand University, Rohtak, Haryana, India
  • Bharat Singh Centre for Biotechnology, Maharshi Dayanand University, Rohtak, Haryana, India
  • Anil Kumar Chhillar Centre for Biotechnology, Maharshi Dayanand University, Rohtak, Haryana, India

Keywords:

Anti-Aspergillus, Bacillus amyloliquefaciens, Toxicity, Aspergillus species, Scanning electron microscopy

Abstract

Objective: Bacteria are able to synthesize a wide range of metabolites with fungicidal properties. The present study focused on the in vitro evaluation of antifungal potential of amyloliquefaciens DSM-1067 against Aspergillus spp. and its electron microscopic studies.

Methods: An invitro evaluation of antifungal activity of bacterial secreted and cellular proteins was determined by microbroth dilution, disc diffusion and spore germination inhibition assays (SGIA). The cytotoxicity of these bacterial proteins was determined by hemolytic assay, and the effect of Bacillus amyloliquefaciens DSM 1067 lysate proteins on Aspergillus fumigatus was visualized by scanning electron microscopy (SEM).

Results: Bacillus amyloliquefaciens DSM-1067 lysates showed the highest activity by inhibiting the growth of A. fumigatus, A. flavus completely at a concentration of 31.25 µg/ml. In vitro toxicity experiments resulted that the lysate of B. amyloliquefaciens DSM-1067was non-toxic against human erythrocytes even at high concentrations. These findings thus emphasize its usefulness in the development of new antifungal therapies. SEM analysis demonstrated the in vitro inhibition of A. fumigatus growth by B. amyloliquefaciens DSM-1067cytosolic proteins leading to wrinkled hyphae, irregular branching patterns. It also showed disruption of conidiophores development. These cytological effects of B. amyloliquefaciens DSM-1067on the hyphal growth of A. fumigatus can explain its potent anti-Aspergillus activity.

Conclusion: The present investigation revealed that B. amyloliquefaciens DSM-1067 lysate protein can act as a potential candidate for exploration in the development of effective and non-toxic treatments against Aspergillus induced diseases. Its effect on the development of conidiophores and hyphal growth are studied in the present study.

 

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Author Biographies

Anu Singh, Centre for Biotechnology, Maharshi Dayanand University, Rohtak, Haryana, India

Research Scholar

Centre for Biotechnology

Meenakshi Balhara, Centre for Biotechnology, Maharshi Dayanand University, Rohtak, Haryana, India

Women Scientist

Centre for Biotechnology

Anil Kumar Chhillar, Centre for Biotechnology, Maharshi Dayanand University, Rohtak, Haryana, India

Associate professor

Centre for Biotechnology

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Published

01-07-2015

How to Cite

Singh, A., M. Balhara, B. Singh, and A. K. Chhillar. “IN VITRO EVALUATION OF ANTIFUNGAL POTENTIAL AND ELECTRON MICROSCOPIC STUDIES OF BACILLUS AMYLOLIQUEFACIENS AGAINST ASPERGILLUS SPECIES”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 7, no. 7, July 2015, pp. 316-20, https://www.innovareacademics.in/journals/index.php/ijpps/article/view/5744.

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