IN VITRO ANTIOXIDANT PROPERTIES AND TOTAL PHENOLIC AND FLAVONOID CONTENTS OF RUMEX VESICAIUS L.
Keywords:
R vesicarius, Free radical scavenging activity, Peroxidase, FRAP, LPO, DPPH, ABTS, MethanolAbstract
Objective: The aim of the study was to investigate the total phenolic and total flavonoids contents and evaluate the antioxidant potential of different solvent extracts of Rumex vesiarius L.
Methods: The antioxidant potential of the hexane, ethyl acetate and methanol extract by using FRAP, LPO and Peroxidase assays and free radical scavenging by using DPPH and ABTS were determined. These activities were determined by using standard protocols with some modifications. Aluminium chloride colorimetric method was used to estimate TFC (total flavonoid content) and TPC (total phenolic content) was measured by Folin-ciocalteu method.
Results: Among the test extracts methanol extract exhibited strong antioxidant activity than that of hexane and ethyl acetate. Free radicals, DPPH and ABTS were significantly inhibited by methanol extract of leaf and fruit (IC50 174.91±17.96 μg/ml and 526.791±91.85 mM min-1g-1DW), whereas methanolic extract of leaves showed good antioxidant potential using FRAP (306±14.8 mM min-1g-1DW) and LPO (30.57±5.65 MDA g-1DW) methods, while Peroxidase was were strongly inhibited by ethyl acetate extract of flower (0.624±0.013 mM min-1g-1DW). The maximum total phenolic and total flavonoids content was observed in leaves (0.53±0.31 mg GAE/gm DW and 2.15±0.72 mg QE/gm DW).
Conclusion: The present investigation suggested that methanol extracts of Rumex vesicarius has significant antioxidant activity. These results clearly indicate that R. vesicarius is effective against free radical mediated diseases. These crude extracts can further purify and may be considered as a new source for antioxidant pharmaceutical compounds.
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