CHEMICAL COMPOSITION AND ANTIFUNGAL ACTIVITY OF ESSENTIAL OIL FROM SATUREJA CALAMINTHA NEPETA AGAINST PHYTOPATHOGENS FUNGI
Keywords:Satureja calamintha nepeta, Essential oils, Antifungal activity, Aspergillus sp, Fusarium Sp
Objective: The increasing incidence of resistance among pathogen towards synthetic fungicides is a cause of serious problem. For this, the fight against fungi is directed to the use of natural products. The main objectives of this study were to investigate the chemical composition and antifungal activity of the essential oil against two post harvest pathogenic fungi: fusarium. sp and Aspergillus. sp and then compare it with the effect of two fungicides (Vapcotop and Propineb) on the same stem.
Methods: Essential oil samples of Satureja calamintha nepeta were analyzed using a Gas Chromatography/Mass Spectrometry (GC-MS) technique. The in vitro antifungal activity of the essential oils was done by the poisoned food technique.
Results: The GCâ€“MS analysis of the essential oil identified 35 components accounting for 99.40% of the total oil composition. The most abundant components were Menthone (26.46%), piperitone oxide (22.26%), and pulegone (14.04%). Essential oil was found effective against all the tested fungi, and was more potent then the two fungicides with minimum inhibitory concentration (MIC) value of 2 Âµl/ml.
Conclusion: The essential oil exhibited a significant reduction in mycelial growth with the two fungi species, and has a higher biological activity than those of the two fungicides. This study suggests that essential oil represents a good alternative to eliminate fungi that can be pathogens for plants.
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