QUALITATIVE AND QUANTITATIVE DETERMINATION OF SECONDARY METABOLITES AND ANTIOXIDANT POTENTIAL OF FICUS BENGHALENSIS LINN SEED
Keywords:
Ficus benghalensis, Phytochemical, Antioxidant, Scavenging, Free radicals, DPPH, Nitric oxide, Lipid peroxidationAbstract
Objective: To evaluate the phytochemical constituents and the antioxidant activity of ethanolic extract of Ficus benghalensis seed locally prescribed as a diet for peptic ulcer.
Methods: Qualitative analysis of various phytochemical constituents and quantitative analysis of total phenolics, flavonoids and tannins were determined by the well-known test protocol available in the literature. The antioxidant and free radical scavenging activity of ethanolic extract of the plant was assessed against DPPH, nitric oxide, lipid peroxidation and reducing power assay using standard protocols.
Results: Phytochemical analysis revealed the presence of phenols, flavones, tannins, carbohydrates, saponins, alkaloids, cardiac glycosides, steroids, quinones, terpenoids and coumarins. The total tannin content of the ethanolic seed extract was high (127.121.508 TEg), followed by phenolics (80.461.868 GEg) and flavonoids (39.231.205 QEg). The activities of plant extract against DPPH, nitric oxide and lipid peroxidation were concentration dependent with IC50 values of 446.9, 596.0 and 557.0 µg respectively. The reducing ability of F. benghalensis was found to be 418.34 at 1000 μg.
Conclusion: Our findings provide evidence that the crude extract of Ficus benghalensis is a potential source of natural antioxidants, and this justifies its use in folkloric medicine.
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Halliwell B, Gutteridge JMC. Free radicals in biology and medicine. UK: Oxford University Press; 1999.
Yildirim A, Mavi A. Comparison of antioxidant and antimicrobial activities of Tilia argenta, Salvia triloba and Camelia sinesis extracts. J Agric Food Chem 2000;48:5030-4.
Gulcin I, Oktay MO. Determination of antioxidant activity of Lichen Cetraria islandica. Ach J Ethanopharmacol 2002;79:325-9.
Kourornakis AP, Galanakis DK. Synthesis and pharmacological evaluation of novel derivatives of anti-inflammatory drugs with increased antioxidant and anti-inflammatory activities. Drug Dev Res 1999;47:9-16.
Gulcin I, Buyukokuroghee ME. On the in vitro antioxidant properties of melatonin. J Pineal Res 2002;33:167-71.
Halliwell B, Gutteridge JMC. Free radicals, other reactive species and disease. In: Free radical in biology and medicine. Oxford: Clarendron press; 1999. p. 617.
Hyoung LS. The antioxidant activity of the browning reaction product isolated from storage-aged orange juice. J Agric Food Chem 1992;40:550-2.
Branen AL. Toxicology and biochemistry of butylated hydroxyanisole and butylated hydroxytoluene. J Am Oil Chem Soc 1975;52:59-63.
Zheng W, Wang SY. Antioxidant activity and phenolic compounds in selected herbs. J Agric Food Chem 2001;49:5165-70.
Cai YZ, Sun M, Corke H. Antioxidant activity of betalains from plants of the Amaranthaceae. J Agric Food Chem 2003;51:2288-94.
Sala A, Recio MD, Giner RM, Manez S, Tournier H, Schinella G, et al. Anti-inflammatory and antioxidant properties of Helichrysum italicum. J Pharm Pharmacol 2002;54:365-71.
Rice ECA, Miller NJ, Bolwell PG, Bramley PM, Pridham JB. The relative activities of Plant-derived polyphenolic flavonoid. Free Radical Res 1995;22:375-83.
Ashokkumar D, Mazumder UK, Gupta M, Senthilkumar GP, Selvan VT. Evaluation of antioxidant and free radical scavenging activities of Oxystelma esculentum in various in vitro models. J Complementary Integr Med 2008;5:9-10.
Veerapur VP, Prabhakar KR, Parihar VP, Kandadi MR, Ramakrishana S. Ficus racemosa stem bark extract: A potent antioxidant and a probable natural radioprotector. Evidence-Based Complementary Altern Med 2009;6:317-24.
Mukherjee PK, Sahu M, Suresh B. Indian herbal medicines. East Pharm 1998;490:21-3.
Gupta RM, Bhise SB, Chandak JT, Kapoor BK. Active constituents of medicinal plants and evolution of synthetic drug. East Pharm 1981;12:39-42.
Nadkarni K. Indian materia medica. Vol. 1. Bombay: Popular Prakashan publication; 1999. p. 543.
Balick M, Paul J, Cox A. Plants that heal; people and culture. Sci Ethnobotany 1996;73:25-61.
Shulz, Volker, Rudolf, Hansel, Mark, Blumenthal. Medicinal plants, phytomedicines and phytotheropy: A physician’s guide to herbal medicine. New York; 2001;4:1-39.
WHO. Good agricultural and field collection practices of medicinal plants. Geneva: Switzerland; 2004. p. 120.
Sanchez-Moreno C. Methods used to evaluate the free radical scavenging activity in foods and biological systems. Food Sci Tech Int 2002;8:121-37.
Harbone JB. Phytochemical methods. London: Chapman and Hall; 1973.
McDonald S, Prenzler PD, Autolovich M, Robards K. Phenolic content and antioxidant activity of olive oil extracts. Food Chem 2001;73:73-84.
Chang C, Yang M, Wen H, Chern J. Estimation of total flavonoid content in Propolis by two complementary calorimetric methods. J Food Drug Anal 2002;10:178-82.
Schanderl SH. Methods in food analysis. New York: Academic Press; 1970. p. 709.
Koleva II, Van Beek TA, Linssen JP, De Groot A, Evstatieva LN. Screening of plant extracts for antioxidant activity: A comparative study of three testing methods. Phytochem Anal 2002;13:8-17.
Green LC, Wagner DA, Glogowski J, Skipper PL, Wishnok JS, Tannenbaum SR. Analysis of nitrate, nitrite and nitrate in biological fluids. Anal Biochem 1982;126:131-8.
Okhawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 1979;95:351-8.
Oyaizu M. Studies on the product of browning reaction prepared from glucose amine. Japan J Nutr 1986;44:307-15.
Orhan I, Kupeli E, Sener B, Yesilada E. Appraisal of anti-inflammatory potential of the Lycopodium cuvatum. Ethnopharmacol 2007;109:146-50.
Rupasinghe HP, Jackson CJ, Poysa V, Di Berado C, Bewley JD, Jenkinson J. Soyasapogenol A and B distribution in soybean in relation to seed physiology, genetic variability and growing location. J Agric Food Chem 2003;51:5888-94.
Srivastava M, Kumar A, Pal M. Phytochemical investigation on Jatropha curcas seed cake. Int J Pharm Life Sci 2010;1:357-62.
Ogunlowo OP, Arimah BD, Adebayo MA. Phytochemical analysis and comparison of in vitro antimicrobial activities of the leaf, stem bark and root bark of F. benghalensis. IOSR J Pharm 2013;3:33-8.
Babu Faten MM. Phytochemical study of F. benghalensis bark. Bull Fac Pharm 2002;40:249-58.
Neena Unnikrishnan, Rinku Mathappan, Manu Somanath, Sheela. Preliminary phytochemical analysis of bark of F. benghalensis and antibacterial activity of its bark oil. RJPBCS 2012;3:388-92.
Priti Diwan I, Yashashree A, Gadhikar. Phytochemical composition and inhibition of oral pathogens by Ficus benghalensis root extracts. Int J Pharm Pharm Sci 2014;6:111-4.
Abdel-Hameed ESS. Total phenolic contents and free radical scavenging activity of certain Egyptian Ficus species leaf samples. Food Chem 2009;114:1271–7.
Nidhi Chowdhary, Mohanjit Kaur, Amarjeet Singh, Bimlesh Kumar. The wound healing activity of aqueous extracts of Ficus religiosa and Ficus benghalensis leaves in rats. IJRPB 2014;2:1071-81.
Yogesh Chand Yadav, Srivastava, Vipin Saini, Sarita Singhal, Seth Sharad Kumar. In vitro antioxidant activity of methanolic extraction of Ficus benghalensis Latex. Pharmacol Online 2011;1:140-8.
Rice-Evans CA, Miller NJ, Paganga G. Structure, antioxidant activity relationships of flavonoids and phenolic acids. Free Radic Biol Med 1996;20:933-56.
Sharma RK, Chatterji S, Rai DK, Mehta S, Rai PK, Singh RK, et al. Antioxidant activities and phenolic contents of the aqueous extracts of some Indian medicinal plants. J Med Plants Res 2009;3:944–8.
Bandekar H, Nagavekar V, Lele S. Studies on Ficus benghalensis: Characterization of fruits and callus induction. J Sci Ind Res 2013;72:553-7.
Xia DZ, Yu XF, Zhu ZY, Zou ZD. Antioxidant and antibacterial activity of six edible wild plants (Sonchus spp.) in China. Nat Prod Res 2011;25:1893–901.
Hausteen B. Flavonoids, a class of natural products of high pharmacological potency. Biochem Pharm 1983;32:1141-8.
Atmani D, Chaher N, Berboucha M, Debbache N, Boudaoud H. Flavonoids in human health: From structure to biological activity. Curr Nutr Food Sci 2009;5:225-37.
Brunetti C, Di Ferdinando M, Fini A, Pollastri S, Tattini M. Flavonoids as antioxidants and developmental regulators: Relative significance in plants and humans. Int J Mol Sci 2013;14:3540-55.
Wu J, Wan Z, Yi J, Wu Y, Peng W, Wu J. Investigation of the extracts from Bidens pilosa for antioxidant activities and cytotoxicity against human tumor cells. J Nat Med 2013;67:17–26.
Koleckar V, Kubikova K, Rehakova Z, Kuca K, Jun D, Jahodar L. Condensed and hydrolysable tannins as antioxidants influencing the health. Mini Rev Med Chem 2008;8:436-47.
Li H, Wang Z, Liu Y. Review of the studies on tannin activity in cancer prevention and anticancer. Zhong-Yao-Cai 2003;26:444-8.
Gayathri M, Kannabiran K. Antimicrobial activity of Hemidesmus indicus, Ficus benghalensis and Pterocarpus marsupium Roxb. Indian J Pharm Sci 2009;71:57-9.
Mabel Parimala, Francis Gricilda Shoba. Phytochemical analysis and in vitro antioxidant activity of hydroalcoholic seed extract of Nymphaea nouchali Burm. Asian Pac J Trop Biomed 2013;3:887-95.
Herin Sheeba Gracelin D, John De Britto A, Benjamin Jeyarathna Kumar P. Qualitative and quantitative analysis of phytochemicals in five Pteris species. Int J Pharm Phar Sci 2013;5:105-7.
Brewer MS. Natural antioxidants: Sources, compounds, mechanisms of action, and potential applications. Compr Rev Food Sci Food Saf 2011;10:221-47.
Halliwell B, Gutteridge JMC. Free radicals in biology and medicine. UK: Oxford University Press; 2007.
Erel SB, Reznicek G, Åženol SG, YavaÅŸoÄŸulu NUK, KonyalioÄŸlu S, Zeybek AU. Antimicrobial and antioxidant properties of Artemisia L. species from Western Anatolia. Turk J Biol 2012;36:75-84.
Karabegovic I, Nikolova M, Velickovic D, Stojicevic S, Veljkovic V, Lazic M. Comparison of antioxidant and antimicrobial activities of methanolic extracts of the Artemisia sp. recovered by different extraction techniques. Chin J Chem Eng 2011;193:504-11.
Deba F, Xuan TD, Yasuda M, Tawata S. Chemical composition and antioxidant, antibacterial and antifungal activities of the essential oils from Bidens pilosa Linn. Food Control 2008;19:346–52.
Madhusudhanan N, Lakshmi T, Kumar SG, Ramakrishnan, Konda VGR, Roy A. In vitro antioxidant and free radical scavenging activity of aqueous and ethanolic flower extract of Nymphaea alba. Int J Drug Dev Res 2011;3:252-8.
Trevisan M, Browne R, Ram M, Muti P, Freudenheim J, Carosella AM. Correlates of markers of oxidative status in the general population. Am J Epidemiol 2001;154:348-56.
Kulisic T, Radonic A, Katalinic V, Milosa M. Use of different methods for testing anti-oxidative activity of oregano essential oil. Food Chem 2004;85:633-40.
Olayinka A, Aiyegoro AI, Okoh. Preliminary phytochemical screening and In vitro antioxidant activities of the aqueous extract of Helichrysum longifolium. BMC Complementary Altern Med 2010;8:10-21.
Anjali Soni, Sheetal Sosa. Phytochemical analysis and free radical scavenging potential of herbal and medicinal plant extracts. JPP 2013;2:2-29.