HOMOCASTASTERONE: A NOVEL PLANT KETOSTEROID INDUCING HAEMATOLOGICAL CHANGES IN NORMAL AND DIABETIC MALE RAT
Keywords:
Homocastasterone, Oxysterol, Haematological parameters, Blood glucose, Lipid peroxidationAbstract
Objective: To study the effect of brassinosteroid keto isoform homocastasterone, in diabetic male wistar rat as an antihyperglycemic factor and to evaluate its effects on the hemodynamic parameters in rat blood.
Methods: Diabetes was induced in a group (n=6) of rats with a single peritoneal injection of streptozotocin at 60 mg/kg. bw. With a treatment schedule of 15 consecutive days, control (n=6) and diabetic rats received 666µg/kg bw, of homocastasterone. Circulating blood glucose, cell count, cell indices, and MDA level was assessed.
Results: Significant reduction (p<0.05) in blood glucose level and increase inRBCs, WBCs, granulocytes, lymphocytes, monocytes and platelets count(p<0.05) along with improved functional indices for HCT, PCV, MCV, MCH, MCHC, MPV, PDW, PCT in homocastasterone treated diabetic group was noted. A significant reduction in RBC-MDA level (p<0.001) in the treated group was noted.
Conclusion: It is suggested that brassinosteroid keto isoform homocastasterone exhibits antiglycemic effect in diabetic rat, and improves RBC, WBC, Platelet counts, haemoglobin level, and cell indices, while reducing peroxidative cell damage in RBCs.
Â
Downloads
References
Gruszka D. The brassinosteroid signaling pathway-new key players and interconnections with other signaling networks crucial for plant development and stress tolerance. Int J Mol Sci 2013;14(5):8740-74.
Davison GP. Effects of a brassinosteroid analogue to mosquito larvae. Ecotoxicol Environ Saf 2003;56(3):419-24.
Morshed M. The effect of plant hormone indoleacetic acid (IAA) on hematological and biochemical parameters in mice. Bangladesh J Physiol Pharmacol 2006;22(1):5-8.
Muthuraman P. Effect of phytohormones on tissue hexokinase and on some blood components in wistar rats. Int J Drug Delivery 2011;2(2):168–72.
Kumar GN, S Lakshmy, K Srikumar. Negative modulation of alkaline phosphatase and creatine kinase by homobrassinolide. Int J Drug Delivery 2011;2(3):258-64.
Muthu S. Acute effect of gibberellic acid on serum enzymes and blood markers in male albino rats. Int J Drug Delivery 2011;3(2):340-7.
Brownlee M, A Cerami, H Vlassara. Advanced glycosylation end products in tissue and the biochemical basis of diabetic complications. N Engl J Med 1988;318(20):1315-21.
Saba AB, AA Oyagbemi, OI Azeez. Antidiabetic and haematinic effects of Parquetina nigrescens on alloxan induced type-1 diabetes and normocytic normochromic anaemia in Wistar rats. Afr Health Sci 2010;10(3):276-82.
Tanko, Y. Anti-diabetic and some haematological effects of ethylacetate and n-butanol fractions of Indigofera pulchra extract on alloxan-induced diabetic Wistar rats. J Diabetes Endocrinol 2011;2(1):1-7.
Mohammed A. Anti-diabetic and some haematological effects of ethylacetate and n-butanol fractions of Ganoderma lucidum aqueous extract in alloxan-induced diabetic wistar rats. Int J Med Med Sci 2009;1(12):530-5.
Epstein FH, NS Young, J Maciejewski. The pathophysiology of acquired aplastic anemia. N Engl J Med 1997;336(19):1365-72.
Wang, Z. Estimation of the normal range of blood glucose in rats. Weisheng Yanjiu 2010;39(2):133-7, 142.
Azeez OI. Ameliorative effects of Cnidoscolus aconitifolius on alloxan toxicity in Wistar rats. Afr Health Sci 2010;10(3):283-91.
Abel-Salam BK. Immunomodulatory effects of black seeds and garlic on alloxan-induced Diabetes in albino rat. Allergol Immunopathol 2012;40(6):336-40.
Ikewuchi JC. Effect of aqueous extract of the leaves of Acalypha wilkesiana ‘Godseffiana’Muell Arg (Euphorbiaceae) on the hematology, plasma biochemistry and ocular indices of oxidative stress in alloxan induced diabetic rats. J Ethnopharmacol 2011;137(3):1415-24.
Han L. Blood coagulation parameters and platelet indices: changes in normal and preeclamptic pregnancies and predictive values for preeclampsia. PLoS One 2014;9(12):e114488.
Boriboonhirunsarn D, R Atisook, T Taveethamsathit. Mean platelet volume of normal pregnant women and severe preeclamptic women in Siriraj Hospital. J Med Assoc Thai 1995;78(11):586-9.
Bath PM, RJ Butterworth. Platelet size: measurement, physiology and vascular disease. Blood Coagulation Fibrinolysis 1996; 7(2):157-61.
Jackson SR, JM Carter. Platelet volume: laboratory measurement and clinical application. Blood Rev 1993;7(2):104-13.
Giovanetti TV, AJ do Nascimento, JP de Paula. Platelet indices: laboratory and clinical applications. Rev Bras Hematol Hemoter 2011;33(2):164-5.