• Mani Deepa Indupalli Department of Botany and Microbiology Acharya Nagarjuna University Guntur, AP India
  • Vijayalakshmi Muvva Department of Botany and Microbiology Acharya Nagarjuna University Guntur, AP India
  • Rajesh Kumar Munaganti Department of Botany and Microbiology Acharya Nagarjuna University Guntur, AP India


Mangrove ecosystems, Phylogenetic study, Streptomyces cellulosae, Bioactive compounds


Objectives: The aim of the present study was to isolate, identify and analyze the phylogenetic characteristics of the potent actinobacterial strain VJDS-1 with antagonistic activities isolated from Mangrove ecosystems of Nizampatnam, Guntur Dist, A.P., India.

Methods: Soil samples collected were pre treated with calcium carbonate and used for isolation of potent actinobacterial strain designated as VJDS-1. Identification of the strain was carried out by studying the micro morphological, cultural, biochemical and physiological methods. The Phylogenetic study of the strain was carried out by employing 16S rDNA sequence based analysis. Phylogenetic tree was constructed using the MEGA (Molecular Evolutionary Genetic Analysis) software version 6.

Results: The potent actinobacterial strain was identified as Streptomyces cellulosae VJDS-1 and the bioactive metabolites produced by the strain inhibited Gram positive bacteria (Staphylococcus aureus, Bacillus megaterium), Gram negative bacteria (Xanthomonas campestris, Proteus vulgaris, Pseudomonas aeruginosa and Escherichia coli) and fungi (Aspergillus niger, Botrytis cinerea, Fusarium solani F. oxysporum and Candida albicans).

Conclusion: The results of the experiment showed that the crude ethyl acetate extract of Streptomyces cellulosae VJDS-1 showed significant antimicrobial potential hence it can be used for isolation of compounds with pharmaceutical importance.



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Tanaka Y, Omura S. Agroactive compounds of microbial origin. Annu Rev Microbiol 1993;47:57-87.

Euzeby JP. List of Prokaryotic names with Standing in Nomenclature.

Berdy J. Thoughts and facts about antibiotics: where we are now and where we are heading. J Antibiot 2012;65:385-95.

Kino T, Hatanaka H, Miyata S, Inamura N, Nishiyama M, Yajima T, et al. FK-506, a novel immune suppressant isolated from a Streptomyces. II. Immunosuppressive effect of FK-506 in vitro. J Antibiot 1987;40:1256-65.

Burg RW, Miller BM, Baker EE, Birnbaum J, Currie SA, Hartman R, et al. Avermectins, new family of potent anti helmintic agents: producing organism and fermentation. Antimicrob Agents Chemother 1979;15:361-7.

Hata T, Hosi T, Kanamori K, Matsumae A, Sano Y, Shima T, et al. Mitomycin, a new antibiotic from Streptomyces. J Antibiot (Tokyo) 1956;9:141-6.

Challis GL, Hopwood DA. Synergy and contingency as driving forces for the evolution of multiple secondary metabolite production by Streptomyces species. Proc Natl Acad Sci USA 2003;100:14555-61.

Fajardo A, Martınez JL. Antibiotics as signals that trigger specific bacterial responses. Curr Opin Microbiol 2008;11:161-7.

Madigan MT, Martinko JM. In: Brock biology of microorganisms. 11th ed. New Jersey: Pearson Education, Upper Saddle River; 2006.

Schrey SD, Tarkka MT. Friends and foes: Streptomycetes as modulators of plant disease and symbiosis. Antonie Van Leeuwenhoek 2008;94:11-9.

Kurtboke DI, Neller RJ, Bellgard SE. Mesophilic actinomycetes in natural and reconstructed sand dune vegetation zones of Fraser Island. Australia Microb Ecol 2007;54:332-40.

Krishna N, Rajesh kumar M, Vijayalakshmi M. Optimization studies for enhanced bioactive metabolite production by Streptomyces violaceoruber VLK-4 isolated from south coast of Andrapradesh India. Int J Pharm Sci Res 2014;5:1000-8.

Hayakawa M. Studies on the isolation and distribution of rare actinomycetes in soil. Actinomycetologica 2008;22:12-9.

Shirling EB, Gottlieb D. Methods for characterization of Streptomyces species. Int J Syst Bacteriol 1966;16:313-40.

Williams ST, Cross T. Actinomycetes In: Methods in Microbiology, Booth C. Eds. Academic press: London; 1971.

Atta HM, Bayoumi R, El-Sehrawi M, Aboshady A, Al-Huminay A. Biotechnological application for producing some antimicrobial agents by actinomycetes isolates from Al-Khurmah Governorate. Eur J Appl Sci 2010;2:98-107.

Kavitha A, Vijayalakshmi M, Sudhakar P, Narasimha G. Screening of Actinomycete strains for the production of antifungal metabolites. Afr J Microbiol Res 2010;4:27-32.

Pridham TG, Anderson P, Foley C, Lindenfelser L, Hesseltine CW, Benedict RGA. Selection of media for maintenance and taxonomic study of Streptomyces. Antibiot Annu 1957;57:947-53.

Pridham TG, Lyons. Methodologies for Actinomyctetales with reference to Streptomycetes. In: Actinomycete Taxonomy. Diatz A, Thayer DW. Eds. Sim special publication No.6, Arligton, VA; 1980. p. 153-224.

Williams ST, Cross T. Isolation, Purification, Cultivation and preservation of actinomycetes. Methods Microbiol 1971;4:295-334.

Pridham TG, Gottlieb D. The utilization of carbon compounds by some actinomycetales as an aid for species determination. J Bacteriol 1948;56:107-14.

Gordon RE. Some criteria for the recognition of Nocardia madura (Vincent) Blanchord. J Gen Microbiol 1966;45:355-64.

Cowan ST. Cowan and Steel’s manual for the identification of medical bacteria. Cambridge, Univ. Press: 2 nd. Edition; 1974.

Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S. MEGA5: Molecular evolutionary genetics analysis using maximum likelihood, Evolutionary distance, and maximum parsimony methods. Mol Biol Evol 2011;28:2731-9.

Krishna N, Rajesh kumar M, Usha Kiranmayi M, Vijayalakshmi M. Optimization of culture conditions for enhanced antimicrobial activity of Rhodococcus erythropolis VLK-12 Isolated from south coast of andhra Pradesh, India. British Microbiol Res J 2014;4:63-79.

Kavitha A, Vijayalakshmi M. Cultural parameters affecting the production of bioactive metabolites by Nocardia levis MK-VL_113. J Appl Sci Res 2009;5:2138-47.

Kavitha A, Vijayalakshmi M. Optimization and purification of L-asparaginase produced by Streptomyces tendae TK-VL_ 333. Z Naturforsch 2010;65:528-31.

Usha Kiranmayi M, Sudhakar P, Krishna N, Vijayalakshmi M. Influence of cultural conditions for improved production of bioactive metabolites by Streptomyces cheonanensis VUK-A Isolated from coringa mangrove ecosystem. Curr Trends Biotechnol Pharm 2012;6:99-111.



How to Cite

Indupalli, M. D., V. Muvva, and R. K. Munaganti. “STREPTOMYCES CELLULOSAE VJDS-1, A PROMISING SOURCE FOR POTENTIAL BIOACTIVE COMPOUNDS”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 7, no. 7, July 2015, pp. 57-61,



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