TO STUDY THE ROLE OF EXERCISE AND DIETARY MANIPULATION IN OBESE RATS TREATED WITH ORLISTAT
Keywords:Anti-obesity, High-fat diet, Low-calorie diet, Exercise, Orlistat
Objective: The present study was undertaken to evaluate the role of exercise and dietary manipulation in obese rats treated with Orlistat.
Methods: The study was conducted for 16 weeks. High-fat diet (HFD) was used to induce obesity, and once these animals were obese (eight week), manipulation in the diet, exercise, Orlistat and low dose of Orlistat were tested for 8 more weeks. During the treatment period, animals were continued with HFD except for the groups where the low-calorie diet (LCD) was used. The various manipulative options studied to include swimming test (physical exercise), Orlistat (200 mg/kg), low dose Orlistat (65 mg/kg) and low-calorie diet. The parameters were evaluated including Body weight was measured on weekly two days. Biochemical parameters such as HDL, LDL, VLDL, Triglycerides and total cholesterol were measured using diagnostic kits. Blood-glucose level was measured. On 16th week, the animals were sacrificed and their organ weights were measured. Organ weights included the Liver, Left and Right kidney and fat pad weights such as Mesenteric fat, Epididymis fat.
Results: There was a significant reduction in body weight, Triglycerides, total cholesterol, LDL, VLDL, Blood-glucose level organ weights included the Liver, Left kidney and Right kidney and fat pad weights such as Mesenteric fat, Epididymis fat and increased in HDL level of the animals treated with exercise and dietary manipulation and Orlistat.
Conclusion: The present study revealed that [HFD+Orlistat (low dose)+Exercise+LCD] group showed more potential effective than rest of all treatment groups.
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