DEVELOPMENT AND VALIDATION OF UV SPECTROMETRIC METHOD FOR QUANTITATIVE DETERMINATION OF ULIPRISTAL ACETATE
Keywords:
Ulipristal acetate (UPA), UV spectrometry, Validation, QuantitationAbstract
Objective: To develop and validate UV spectrometric method for quantitative determination of ulipristal acetate.
Methods: The solvent selected was methanol and detection was carried out at 302 nm.
Results: Linearity of the proposed method was found to be between 5–20 μg/ml. LOD and LOQ were found to be 0.0062 μg/ml and 0.0187 μg/ml, respectively. The % recovery of the proposed method was found to be 98.83 %-100.32 %. The method was found to be precise as the values of % RSD obtained for both intraday and interday, precision studies were found to be<2.0 %. The method was robust and can be useful for routine analysis of formulations containing ulipristal acetate.
Conclusion: The developed method was found to be simple, sensitive, linear, accurate, precise and robust. The developed and validated method can be used for quantitative determination of ulipristal acetate in bulk drugs and dosage form.
Â
Downloads
References
Ghatak SB, Panchal SJ. Ulipristal acetate-A novel oral emergency contraceptive: Hype or hope. Int J Pharm Sci Rev Res 2010;4(3):152-5.
Jadav SP, Parmar PD. Ulipristal acetate, Aprogesterone receptor modulator for emergency contraception. J Pharmacol Pharmacother 2012;3(2):109-11.
Gemzell–Danielsson, Meng CX. Emergency contraception: Potential Role of Ulipristal Acetate. Int J Womens Health 2010;2:53-6.
Faculty of family planning and reproductive health care clinical effectiveness Unit. Emergency contraception. J Family Planning Reprod Health Care 2006;32:121-8.
Fine PM. Ulipristal acetate: a new emergency contraceptive that is safe and more effective than levonorgestrel. Womens Health (Lond Engl) 2011;7(1):9-17.
ICH Harmonized Tripartite Guideline, Validation of analytical procedures: Text and methodology, Q2(R1); 2005. p. 1-13.