• Praful P. Patel Department of Pharmaceutical Sciences, JJT University, Jhunjunu, Rajasthan, India
  • Naitikumar D. Trivedi Department of Pharmacology, A. R., College of Pharmacy, Anand, Gujarat, India


Karanja seed oil, Karanjin, Furanoflavonoid, Pongamol, Diketone


Objective: Study was undertaken with the objective, to develop simple, high yielding and economic method for the isolation as well as analysis of Karanjin and Pongamol from Karanja oil.

Methods: Karanja oil was subjected to extraction with methanol. Methanol extract was washed with little quantity of pet ether for removal of residual oil. Resultant extract was dissolved in a sufficient quantity of methanol and kept aside for 6 hours,a white precipitate formed which was subjected to repeated crystallization to get karanjin (1K). Residue obtains after precipitate removal was concentrated into half and extracted with dilute acetic acid and kept aside for 48 hours. A pale yellow crystals were formed on the side wall,collected it and chromatographed over the column with hexane and ethyl acetate (95:5) to obtain pure Pongamol (2P). Karanjin and Pongamol was analyzed by high performance liquid chromatography (HPLC).

Results: Yield of Karanjin was 35.5 g (0.89 %) and pongamol was 27.3 g (0.68 %) from 4 l of Karanja oil. HPLC method developed for the analysis of karanjin and pongamol produce results which comply with USP standards. Both compounds fail to produce a significant antibacterial effect on gram positive and negative bacteria as well as on pathogenic fungi and yeast.

Conclusion: This method for isolation is simple, economic and gives good yields of karanjin & pongamol from Karanja oil and HPLC method for the analysis of karanjin and pongamol is suitable for simultaneous estimation and reliable.



Download data is not yet available.

Author Biography

Praful P. Patel, Department of Pharmaceutical Sciences, JJT University, Jhunjunu, Rajasthan, India

Pharmaceutical Sciences, Research Scholar


Khare CP. Pongamia pinnata. In: Indian medicinal plants an illustrated dictionary. Springer; 2007. p. 511-2.

Srinivasan K, Muruganandan S, Lal J, Chandra S, Tandan SK, Ravi PV. Evaluation of anti-inflammatory activity of Pongamia pinnata leaves in rats. J Ethnopharmacol 2001;78:151–7.

Samuel AJSJ, Radhamani S, Gopinath R, Kalusalingam A, Vimala AGKA, Husain HA. In vitro screening of anti-lice activity of Pongamia pinnata leaves. Korean J Parasitol 2009;47(4):377-80.

Vadivel V, Biesalski HK. Contribution of phenolic compounds to the antioxidant potential and type II diabetes related enzyme inhibition properties of Pongamia pinnata L. Pierre seeds. Process Biochem 2011;46:1973–80.

Goel RK, Prabha T, Dorababu M, Goel S, Agrawal PK, Singh A, et al. Effect of methanolic extract of Pongamia pinnata Linn seed on gastro-duodenal ulceration and mucosal offensive and defensive factor in rats. India J Exp Biol 2009;47:649-59.

Nirmal SA, Malwadkar G, Laware RB. Anthelmintic activity of Pongamia glabra. Songklanakarin. J Sci Technol 2007;29(3):755-7.

Dharmesh SM, Vismaya, Belagihally SM, Rajashekhar S, Jayaram VB, Kanya S, et al. Gastroprotective properties of karanjin from karanja (Pongamia pinnata) seeds; role as antioxidant and H+, K+-ATpase inhibitor. Evidence-Based Complementary Altern Med 2011;20:1-10.

Jorgensen JH, Turnidge. Susceptibility test methods: dilution and disk diffusion methods. In: Manual of clinical Microbiology 9th ed. American society for microbiology; 2007. p. 1152â€73.

Srivastava AK, Tamrakar AK, Yadav PP, Tiwari P, Maurya R. Identification of pongamol and karanjin as lead compounds with antihyperglycemic activity from Pongamia pinnata fruits. J Ethnopharmacol 2008;118:435–9.

Parmar VS, Rathore JS, Jain R, Henderson DA, Malone JF. Occurrence of pongamol as the enol structure in Tephrosiapurpurea. Phytochemistry 1989;28(2):591-3.

Katekhaye S, Kale MS, Laddha KS. Development and validation of the HPLC method for Karanjin in Pongamia pinnataLinn. leaves. Indian J Pharm Sci 2012;74(1):72-5.



How to Cite

Patel, P. P., and N. D. Trivedi. “SIMPLE, EFFICIENT AND ECONOMIC METHOD FORISOLATION AND ANALYSIS OF KARANJIN AND PONGAMOL FROM KARANJA SEED OIL AND SCREENING OF ANTIMICROBIAL POTENTIAL”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 7, no. 7, July 2015, pp. 248-52,



Original Article(s)