INVESTIGATION GENOTOXICITY OF NEW SILVER-BASED COMPLEXWITH ANTIMICROBIAL ACTIVITY
Keywords:Geno toxicity, Comet assay, Silver-based complex, Antibacterial drug, Silver hexa-methylene tetramine
Objective: Investigation of genotoxicity effects of the new antibacterial silver-based compound with of general formula [Ag (CH2)6 N4]+NO3.
Methods: The comet assay applied for assessment of genotoxic effects on leukocytes of mice blood. DNA damage was assessed on the percentage of DNA in a comet tail (tail DNA), under influence of silver complex in different concentrations.
Results: During experiments found a significant increase in the level of DNA damage in cells, at the maximum tested concentrations. At low doses, the drug has no significant genotoxic effect.
Conclusion: Tested silver complex did not cause DNA damage at low concentrations and could be considered as a base for new antibacterial drug.
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