BIO-PROSPECTING THE IN-VITRO ANTIOXIDANT AND ANTI-CANCER ACTIVITIES OF SILVER NANOPARTICLES SYNTHESIZED FROM THE LEAVES OF SYZYGIUM SAMARANGENSE

Authors

  • Nivetha Thampi Department of Biotechnology, Jeppiaar Engineering College, Rajiv Gandhi Salai, Chennai 600119, Tamil Nadu, India
  • J. Veronica Shalini Department of Biotechnology, Jeppiaar Engineering College, Rajiv Gandhi Salai, Chennai 600119, Tamil Nadu, India

Keywords:

Silver nanoparticles, Syzygium samarangense, UV-Visible, FT-IR, FESEM, Antioxidant, Cytotoxic activity, MTT assay

Abstract

Objective: Green nanotechnology involves the tailoring of nanoparticles through a reliable and eco-friendly process making it suitable for a desired application. The current study is focussed on the biosynthesis of silver nanoparticles (AgNPs) using aqueous extract of Syzygium samarangense (Java Apple) leaves and to investigate their total antioxidant capacity (TAC), free radical scavenging activity and the anticancer activity.

Methods: The crude leaf extracts of S. samarangense was used to synthesize the AgNPs from 1 mM silver nitrate solution and the formation of AgNPs was confirmed by UV-Visible spectrophotometer, FT-IR and FESEM techniques. The TAC was determined by phosphomolybdenum method whereas the free radical scavenging activity was studied by H2O2 method. Cytotoxic activity was performed by MTT assay using the AgNPs against A549 cell lines.

Results: Biosynthesis of AgNPs was visually confirmed by observing the colour change from pale yellow to dark brown. UV-Visible spectral analysis showed silver Surface Plasmon Resonance band at 425 nm and the FT-IR peaks showed the presence of proteins and phenolic groups that are responsible for the stabilization of AgNPs. FESEM image showed the presence of AgNPs that were spherical shaped and poly dispersed. The efficiency of AgNPs as a source of good antioxidant and as a potential free radical scavenger was confirmed from the results of TAC and H2O2 assay. Further these nanoparticles showed reduced viability and increased cytotoxicity on A549 cell line in a dose dependent manner.

Conclusion: The present investigation suggests an impressive method for the biological reduction of silver to silver nanoparticles that can be fabricated into many valuable and replaceable therapeutic agents in the treatment of various lethal diseases.

 

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Author Biographies

Nivetha Thampi, Department of Biotechnology, Jeppiaar Engineering College, Rajiv Gandhi Salai, Chennai 600119, Tamil Nadu, India

M.tech Biotechnology

J. Veronica Shalini, Department of Biotechnology, Jeppiaar Engineering College, Rajiv Gandhi Salai, Chennai 600119, Tamil Nadu, India

Associate Professor, Department of Biotechnology

References

Hutchison JE. Greener nanoscience: a proactive approach to advancing applications and reducing implications of nanotechnology. ACS Nano 2008;2:395–402.

Leela A, Vivekanandan M. Tapping the unexploited plant resources for the synthesis of silver nanoparticles. Afr J Biotechnol 2008;7:3162-05.

Thirumurgan A, Tomy N, Jai Ganesh R, Gobikrishnan S. Biological reduction of silver nanoparticles using plant leaf extracts and its effect an increased antimicrobial activity against clinically isolated organism. De Phar Chem 2010;2:279-84.

Parashar V, Parashar R, Sharma B, Pandey AC. Parthenium leaf extract mediated synthesis of silver nanoparticles: a novel approach towards weed utilization. Digest J Nanomater Biostructures 2009;4:45-50.

Geoprincy G, Vidhya Srri BN, Poonguzhali U, Nagendra Gandhi N, Renganathan S. A review on green synthesis of silver nanoparticles. Asian J Pharm Clin Res 2013;6(1):8-12.

Park Y, Hong YN, Weyers A, Kim YS, Linhardt RJ. Polysaccharides and phytochemicals: a natural reservoir for the green synthesis of gold and silver nanoparticles. IET Nanobiotechnol 2011;5(3):69–78.

Iravani S. Green synthesis of metal nanoparticles using plants. Green Chem 2011;13:2638-50.

Narayanan KB, Sakthivel N. Green synthesis of biogenic metal nanoparticles by terrestrial and aquatic phototrophic and heterotrophic eukaryotes and biocompatible agents. Adv Colloid Interface 2010;156:1-13.

Abilash G, Podila R, Karanam L, Chelli J, Rao AM. Catalytic reduction of 4-nitrophenol using biogenic gold and silver nanoparticles derived from breynia rhamnoides. Langmuir 2011;27:15268–74.

Santhoshkumar T, Rahuman AA, Rajakumar G, Marimuthu S, Bagavan A, Jayaseelan C, et al. Synthesis of silver nanoparticles using Nelumbo nucifera leaf extract and its larvicidal activity against malaria and filariasis vectors. Parasitol Res 2011;108:693-702.

Allen RD, Webb RP, Schake SA. Use of transgenic plants to study antioxidant defenses. Free Radical Biol Med 1997;23:473–9.

Willcox JK, Ash SL, Catignani GL. Antioxidants and prevention of chronic disease. Crit Rev Food Sci Nutr 2004;44:275-95.

Pham-Huy LA, He H, Pham-Huyc C. Free Radicals, Antioxidants in disease and health. Int J Biomed Sci 2008;4(2):89-96.

Kundu Sen S, Gupta M, Mazumder UK, Haldar PK, Saha P, Bala A. Antitumor activity of Citrus maxima (Burm.) Merr. leaves in Ehrlich's Ascites Carcinoma cell-treated mice. ISRN Pharmacol 2011;1:1-4.

Ahmedin J, Bray F, Center M. Global cancer statistics. CA: Cancer J Clinicians 2011;61:69-90.

Unno Y, Shino Y, Kondo F, Igarashi N, Wang G, Shimura R, et al. Oncolytic viral therapy for cervical and ovarian cancer cells by Sindbis virus AR339 strain. Clin Cancer Res 2005;11:4553-60.

Abraham SA, McKenzie C, Masin D, Ng R, Harasym TO, Mayer LD, et al. In vitro and in vivo characterization of doxorubicin and vincristine coencapsulated within liposomes through use of transition metal ion complexation and pH gradient loading. Clin Cancer Res 2004;10:728-38.

Byrd JC, Lucas DM, Mone AP, Kitner JB, Drabick JJ, Grever MR. KRN5500:a novel therapeutic agent with in vitro activity against human B-cell chronic lymphocytic leukemia cells mediates cytotoxicity via the intrinsic pathway of apoptosis. Blood 2003;101:4547-50.

Amiji MM. editor. Nanotechnology for Cancer Therapy. Taylor and Francis. CRC Press; 2007.

Raphael J, Hicz AH, Souza l. Prognostic factors in squamous cell carcinoma of the oral cavity. Rev Bras Otorhinolaringol 2008;74:861-6.

Vaidyanathan R, Kalishwaralal K, Gopalram S, Gurunathan S. Nano silver-the burgeoing therapeutic molecule and its green synthesis. Biotechnol Adv 2009;27(6):924-37.

Kim JS, Kuk E, Nam K, Kim JH, Park SJ, Leo HJ, et al. Antimicrobial effect of silver nanoparticles. Nanomed 2007;3:95-101.

Lin J, Yan F, Tang L, Chen F. Antitumor effects of curcin from seeds of Jatropha curcas. Acta Pharmacol Sin 2003;24:241–6.

Sahoo SK, Dilnawaz F, Krishnakumar S. Nanotechnology in ocular drug delivery. Drug Discovery Today 2008;13:144–51.

Prieto P, Pineda M, Aguilar M. Spectrophotometric quantitation of Antioxidant capacity through the formation of a Phosphomolybdenum complex: specific application to the determination of vitamin E. Anal Biochem 1999;269:337-41.

Ruch RJ, Cheng SJ, Klaunig JE. Prevention of cytotoxicity and inhibition of intracellular communication by antioxidant catechins isolated from Chinese green tea. Carcinogenesis 1989;10:1003-8.

Mossman T. Rapid colorimetric assay for cellular growth and survival–application to proliferation and cytotoxicity assays. J Immunol Methods 1983;65:55-63.

Sawle BD, Salimath B, Deshpande R, Dhondojirao Bedre M, Murthy Prabhakar BK, Venkataraman A. Biosynthesis and stabilization of Au and Au–Ag alloy nanoparticles by fungus, Fusarium semitectum. Sci Technol Adv Mater 2008;9:1-6.

Jae YS, Beom SK. Rapid biological synthesis of silver nanoparticles using plant leaf extracts. Bioprocess Biosyst Eng 2009;32:79-84.

Sondi I, Salopek-Sondi B. Silver nanoparticles as antimicrobial agent: a case study on E. coli as a model for Gram-negative bacteria. J Colloid Interface Sci 2004;275(1):177-82.

Rai A, Singh A, Ahmad A, Sastry M. Role of halide ions and temperature on the morphology of biologically synthesized gold nanotriangles. Langmuir 2006;22:736-41.

Gole A, Dash C, Ramachandran V, Mandale AB, Sainkar SR, Rao M, et al. Pepsin-gold colloid conjugates: preparation, characterization, and enzymatic activity. Langmuir 2001;17:1674–9.

Rajendran R, Ganesan N, Balu SK, Alagar S, Thandavamoorthy P, Thiruvengadam D. Green synthesis, characterization, antimicrobial and cytotoxic effects of silver nanoparticles using Origanum heracleoticum L. Leaf extract. Int J Pharm Pharm Sci 2015;7:288-93.

Aderogba MA, Okoh EK, Idowu TO. Evaluation of the antioxidant activity of the secondary metabolites from piliostigma reticulatum. Hochst J Biol Sci 2005;5(2):239-42.

Cho KH, Park JE, Osaka T, Park SG. The study of antimicrobial activity and preservative effects of nanosilver ingredient. Electrochim Acta 2005;51:956.

Martins D, Frungillo l, Anazzetti MC. Antitumoral activity of L-ascorbic acid-poly-D, L-(lactide-co-glycolide) nanoparticles containing violacein. Int J Nanomed 2010;5:77-85.

Sriram MI, Kanth SBM, Kalishwaralal K. Antitumor activity of silver nanoparticles in Dalton’s lymphoma ascites tumor model. Int J Nanomed 2010;5:753-62.

Zolghadri S, Saboury A, Golestani A, Divsalar A, Rezaei-Zarchi S, Moosavi-Movahedi A. Interaction between silver nanoparticle and bovine hemoglobin at different temperatures. J Nanopart Res 2009;11:1751-8.

Published

01-07-2015

How to Cite

Thampi, N., and J. V. Shalini. “BIO-PROSPECTING THE IN-VITRO ANTIOXIDANT AND ANTI-CANCER ACTIVITIES OF SILVER NANOPARTICLES SYNTHESIZED FROM THE LEAVES OF SYZYGIUM SAMARANGENSE”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 7, no. 7, July 2015, pp. 269-74, https://www.innovareacademics.in/journals/index.php/ijpps/article/view/6328.

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