BIO-PROSPECTING THE IN-VITRO ANTIOXIDANT AND ANTI-CANCER ACTIVITIES OF SILVER NANOPARTICLES SYNTHESIZED FROM THE LEAVES OF SYZYGIUM SAMARANGENSE
Keywords:Silver nanoparticles, Syzygium samarangense, UV-Visible, FT-IR, FESEM, Antioxidant, Cytotoxic activity, MTT assay
Objective: Green nanotechnology involves the tailoring of nanoparticles through a reliable and eco-friendly process making it suitable for a desired application. The current study is focussed on the biosynthesis of silver nanoparticles (AgNPs) using aqueous extract of Syzygium samarangense (Java Apple) leaves and to investigate their total antioxidant capacity (TAC), free radical scavenging activity and the anticancer activity.
Methods: The crude leaf extracts of S. samarangense was used to synthesize the AgNPs from 1 mM silver nitrate solution and the formation of AgNPs was confirmed by UV-Visible spectrophotometer, FT-IR and FESEM techniques. The TAC was determined by phosphomolybdenum method whereas the free radical scavenging activity was studied by H2O2 method. Cytotoxic activity was performed by MTT assay using the AgNPs against A549 cell lines.
Results: Biosynthesis of AgNPs was visually confirmed by observing the colour change from pale yellow to dark brown. UV-Visible spectral analysis showed silver Surface Plasmon Resonance band at 425 nm and the FT-IR peaks showed the presence of proteins and phenolic groups that are responsible for the stabilization of AgNPs. FESEM image showed the presence of AgNPs that were spherical shaped and poly dispersed. The efficiency of AgNPs as a source of good antioxidant and as a potential free radical scavenger was confirmed from the results of TAC and H2O2 assay. Further these nanoparticles showed reduced viability and increased cytotoxicity on A549 cell line in a dose dependent manner.
Conclusion: The present investigation suggests an impressive method for the biological reduction of silver to silver nanoparticles that can be fabricated into many valuable and replaceable therapeutic agents in the treatment of various lethal diseases.
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