IMMUNOMODULATORY EFFECT OF DIFFERENT PROPORTIONS OF THE HERBAL MIXTURE IN TRIPHALA ON HUMAN T LYMPHOCYTES (MOLT-4)
Keywords:Triphala, Immunomodulatory activity, MOLT-4 cells, Interferon-g, Interleukin-10, Th1Th2 balance
Objectives: To investigate effect of different proportions of Terminalia bellerica Roxb (TB):Terminalia chebula Retz (TC):Phyllanthus emblica L. (PE) in triphala extract on immunological activity and to determine the possibility of applying the appropriate extract for health and immune disorders.
Methods: The extracts with different proportions of TB: TC: PE (w/w/w), i.e.12:8:4 (F1), 4:12:8 (F2), 8:4:12 (F3) and 8:8:8 (F4), were prepared by decoction in water and dried under vacuum. Gallic acid, a major compound in triphala, was detected by high performance liquid chromatography (HPLC). The effect of the extracts on IFN-g and IL-10 cytokine production produced by MOLT-4 cells was determined by ELISA.
Results: The results show that F1, F4 and TB extracts significantly stimulated IFN-g production but no alteration in IL-10 expression was observed. With LPS induction, F1, F2, F3 and PE extracts significantly inhibited IFN-g production, while F2, F3, TB and TC extracts inhibited IL-10 production. By determining the IFN-g/IL-10 cytokine ratio, we found that the Th1/Th2 balance after treatment with triphala extract was mainly skewed toward a Th1-like response. With LPS induction, only the F1 extract could restore the balance of immunity by shifting the Th2 response to a normal level.
Conclusion: Our investigation indicates that different proportions of triphala extracts and induction conditions affect cytokine production, with a predominant Th1 response. F4, the equal proportion triphala extract, could be applied as a healthy herbal drink. F1, containing a high proportion of T. bellerica, was a promising extract as an effective therapeutic intervention against Th2 imbalance diseases such as allergy and autoimmune disease or for use with cancer vaccines.
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