• Aurasorn Saraphanchotiwitthaya Department of Pharmaceutical Technology, Faculty of Pharmaceutical Sciences, Naresuan University, Phitsanulok, Thailand, 2Pharmaceutical Biotechnology Research Unit, Faculty of Pharmaceutical Sciences, Naresuan University, Phitsanulok, Thailand
  • Pattana Sripalakit 2Pharmaceutical Biotechnology Research Unit, Faculty of Pharmaceutical Sciences, Naresuan University, Phitsanulok, Thailand, 3Department of Pharmaceutical Chemistry and Pharmacognosy, Faculty of Pharmaceutical Sciences, Naresuan University, Phitsanulok, Thailand


Triphala, Immunomodulatory activity, MOLT-4 cells, Interferon-g, Interleukin-10, Th1Th2 balance


Objectives: To investigate effect of different proportions of Terminalia bellerica Roxb (TB):Terminalia chebula Retz (TC):Phyllanthus emblica L. (PE) in triphala extract on immunological activity and to determine the possibility of applying the appropriate extract for health and immune disorders.

Methods: The extracts with different proportions of TB: TC: PE (w/w/w), i.e.12:8:4 (F1), 4:12:8 (F2), 8:4:12 (F3) and 8:8:8 (F4), were prepared by decoction in water and dried under vacuum. Gallic acid, a major compound in triphala, was detected by high performance liquid chromatography (HPLC). The effect of the extracts on IFN-g and IL-10 cytokine production produced by MOLT-4 cells was determined by ELISA.

Results: The results show that F1, F4 and TB extracts significantly stimulated IFN-g production but no alteration in IL-10 expression was observed. With LPS induction, F1, F2, F3 and PE extracts significantly inhibited IFN-g production, while F2, F3, TB and TC extracts inhibited IL-10 production. By determining the IFN-g/IL-10 cytokine ratio, we found that the Th1/Th2 balance after treatment with triphala extract was mainly skewed toward a Th1-like response. With LPS induction, only the F1 extract could restore the balance of immunity by shifting the Th2 response to a normal level.

Conclusion: Our investigation indicates that different proportions of triphala extracts and induction conditions affect cytokine production, with a predominant Th1 response. F4, the equal proportion triphala extract, could be applied as a healthy herbal drink. F1, containing a high proportion of T. bellerica, was a promising extract as an effective therapeutic intervention against Th2 imbalance diseases such as allergy and autoimmune disease or for use with cancer vaccines.



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How to Cite

Saraphanchotiwitthaya, A., and P. Sripalakit. “IMMUNOMODULATORY EFFECT OF DIFFERENT PROPORTIONS OF THE HERBAL MIXTURE IN TRIPHALA ON HUMAN T LYMPHOCYTES (MOLT-4)”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 7, no. 7, July 2015, pp. 282-8,



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