BIOLOGICAL ACTIVITY OF BULGARIAN FOLIA BETULAE DRY EXTRACT

Authors

  • Dimitar Penkov Department of Pharmaceutical Sciences, Faculty of Pharmacy, Medical University of Plovdiv, 4000 Plovdiv, Bulgaria
  • Stela Dimitrova Department of Chemistry and Biochemistry, Medical University of Plovdiv, 4000 Plovdiv, Bulgaria
  • Velichka Andonova Medical University of Plovdiv, Bulgaria
  • Emilia Milieva Medical University of Plovdiv, Bulgaria
  • Mariana Murdjeva Medical University of Plovdiv, Bulgaria
  • Irina Stanimirova Medical University of Plovdiv, Bulgaria
  • Marian Draganov Medical University of Plovdiv, Bulgaria
  • Margarita Kassarova Medical University of Plovdiv, Bulgaria

Keywords:

Folia Betulaeextract, HPLC identification, Biological activity

Abstract

Objective: The aim of this study was to investigate the biological activity of dry Folia Betulae (FB) extract.

Methods: Extracts from birch leaves were obtained by different technological methods–maceration and percolation, extraction with different concentrations of ethanol, changes in temperature regimen. The influence of the technological factors on the content of the biologically active substances (BAS) was examined. A phytochemical characterization of the extracts and their standardization were made, according to important groups of BAS–flavonoids (rutin, quercetin) and terpenes (betulin and betulinic acid), by means of HPLC methods for detection and quantitative determination. A model extract, with optimal content of BAS was chosen for subsequent in vitro investigation of its biological activity. Antimicrobial activity was studied via in vitro tests using bacterial isolates–Staphylococcus aureus, Escherichia coli and Candida albicans. The physiological activity was investigated by using in vitro test with smooth muscle strips. The antiproliferative activity of FB extract on eukaryotic cells was examined on cell cultures in vitro. Two cell cultures were used: the mouse lymphoma cell line L5178Y and the serum-free McCoy-Plovdiv cells.

Results: The dry extract from FB has a dose–dependent antibacterial effect. The bactericidal effect on Staphylococcus aureus is stronger than the one on Escherichia coli. Results prove that adding the extract leads to stimulating effect on muscle contractility. It demonstrates biological activity, expressed as changes in cell morphology, proliferation and vitality as well as initiation of apoptosis.

Conclusion: The results obtained largely overlap with literature data and explain some of the applications of this plant in traditional medicine.


 

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Author Biographies

Dimitar Penkov, Department of Pharmaceutical Sciences, Faculty of Pharmacy, Medical University of Plovdiv, 4000 Plovdiv, Bulgaria

assist prof. at Faculty of Pharmacy, department "Pharmaceutical sciences"

Stela Dimitrova, Department of Chemistry and Biochemistry, Medical University of Plovdiv, 4000 Plovdiv, Bulgaria

assist. prof. at Department of Chemistry and Biochemistry

Velichka Andonova, Medical University of Plovdiv, Bulgaria

chief assist. prof. at Department of Pharmaceutical  Sciencesâ€

Emilia Milieva, Medical University of Plovdiv, Bulgaria

assoc. prof. at Department of Medical Physics, Mathematics and Biophysics

Mariana Murdjeva, Medical University of Plovdiv, Bulgaria

prof. at Department of Microbiology and Immunology

Irina Stanimirova, Medical University of Plovdiv, Bulgaria

assist. prof. at Department of Microbiology and Immunology

Marian Draganov, Medical University of Plovdiv, Bulgaria

assoc. prof. at Department of Biology

Margarita Kassarova, Medical University of Plovdiv, Bulgaria

assoc. prof. at Department of Pharmaceutical  Sciencesâ€

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Published

01-07-2015

How to Cite

Penkov, D., S. Dimitrova, V. Andonova, E. Milieva, M. Murdjeva, I. Stanimirova, M. Draganov, and M. Kassarova. “BIOLOGICAL ACTIVITY OF BULGARIAN FOLIA BETULAE DRY EXTRACT”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 7, no. 7, July 2015, pp. 289-94, https://www.innovareacademics.in/journals/index.php/ijpps/article/view/6434.

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