BIOACTIVITY GUIDED FRACTIONATION AND ANTI-INFLAMMATORY ACTIVITY OF ACACIA NILOTICA PODS
Keywords:Acacia nilotica, Anti-inflammatory, Phenolics and HPTLC
Objective: The present study aims at screening the methanol extract and the tannin fraction of the pods of Acacia nilotica for anti-inflammatory activity.
Methods: The methanol extract and its tannin fraction were evaluated for acute toxicity using rats as per the Organization for Economic Co-operation and Development (OECD) guidelines. They were found to be safe up to a dose of 2000 mg/kg. The anti-inflammatory activity of the extract and its fraction was evaluated using the carrageenan-induced rat paw edema method for determining the acute phase of inflammation. The activity against sub-acute inflammation was evaluated using the cotton pellet granuloma pouch method. Diclofenac sodium was used as the reference drug.
Results: The results of pharmacological screening indicated the methanol extract to possess statistically significant anti-inflammatory activity at 100 mg/kg dose. The tannin fraction of methanol extract was found to be more potent than the methanol extract with maximum response at 25 mg/kg dose.
Conclusion: The methanol extract and tannin fraction of pods of Acacia nilotica possess significant anti-inflammatory activity the latter one being more potent.
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