• Sreenath Kunnathupara Bhaskaran Karpagam University
  • Anandan Rangasamy Central Institute of Fisheries Technology
  • Sowmya Sundaram Karpagam University
  • Velliyur Kanniappan Gopalakrishnan Karpgam University
  • Poornima Kannappan Department of Biochemistry, Karpagam University, Coimbatore 641021, Tamil Nadu, India


Azolla filiculoides, Antisecretory, Acid neutralizing, Antioxidant


Objective: To investigate the anti ulcer effect of aqueous extract of Azolla  filiculoides (AF) in experimentally induced gastric ulceration in male Wistar rats.

Methods: Twenty four rats were divided into four groups of six rats in each group. The group I and III rats were fed with standard diet, whereas, group II and IV rats were pre-treated orally with aqueous extract of Azolla microphylla (200 mg/kg body) twice a day for 15 d. Twenty four hours before sacrifice, group III and IV rats were induced with ulcer by pylorus-ligation plus oral administration of a single dose of HCl-ethanol mixture (1.5 ml). The gastric juice and mucosal scraps were collected from all the groups for biochemical analyses.

Results: In ulcer induced rats, there observed a significant (P<0.05) increase in the following parameters like ulcer index, gastric output, acid output, lipid peroxides and these levels were significantly (P<0.05) reduced to near normal values in AF pre-treated rats. A significant decrease was also observed in the levels of reduced glutathione, hexose, hexosamine, sialic acid and in the activities of antioxidant enzymes (glutathione-S-transferase and glutathione peroxidase) and antiperoxidative enzymes (catalase and superoxide dismutase) in ulcer induced rats. These values were restored back to near normalcy in AF pre-treated rats.

Conclusion: The results reinforce the antisecretory, acid neutralizing and the antioxidant potential of the whole plant extract of AF against experimentally induced gastric ulcer in rats. However, further studies are needed to identify the active principle involved in eliciting the antiulcer activity of the plant.



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Author Biography

Poornima Kannappan, Department of Biochemistry, Karpagam University, Coimbatore 641021, Tamil Nadu, India

Associate professor, Department of Biochemistry


Take S, Mizuno M, Ishiki K, Nagahara Y, Yoshida T, Yokota K, et al. The effect of eradicating Helicobacter pylori on the development of gastric cancer in patients with peptic ulcer disease. Am J Gastroenterol 2005;100:1037-42.

Manjari V, Das UN. Effect of polyunsaturated fatty acids on dexamethasone induced gastric mucosal damage. Prostaglandins Leukotrienes Essent Fatty Acids 2000;62:85-96.

Gyires K. Gastric mucosal protection from prostaglandins to gene-therapy. Curr Med Chem 2005;12:203-15.

Srinivas TL, Lakshmi SM, Shama SN, Reddy GK, Prasanna KR. Medicinal plants as Anti-Ulcer agents. J Pharmacogn Phytochem 2013;2:91-7.

Sharma RK, Mandal S, Rajani GP, Gupta N, Srivastava DP. Antiulcer and Anti-inflammatory activity of fresh leave extracts of Polyalthia longifolia in rats. Int J Drug Dev Res 2011;3:351-9.

Sayanti B, Susri RC, Subrata C, Sandip KB. Healing properties of some Indian medicinal plants against Indomethacin-induced gastric ulceration of rats. J Clin Biochem Nutr 2007;41:106-14.

Noor Nawaz AS, Syed J, Dileep N, Rakesh KN, Prashith Kekuda TR. Antioxidant activity of Azolla pinnata and Azolla rubra-A comparative study. Sch Acad J Biosci 2014;2:719-23.

Abraham G. Evaluation of antimicrobial activity of methanolic extracts of Azolla microphylla. Vegetos-An Int J Plant Res 2013;26:200-4.

Pereira AL, Bessa LJ, Leão PN, Vasconcelos V, da Costa PM. Bioactivity of Azolla aqueous and organic extracts against bacteria and fungi. Symbiosis 2015;65:17-21.

Kumar A, Kumari J, Kumar H, Nath A, Singh JK, Ali M, et al. Hepatoprotective and antioxidant effect of Azolla filiculoides on Profenofos induced hepatotoxicity in Swiss Albino mice. Carib J Sci Tech 2014;2:372-7.

Hara N, Okabe S. Effects of gefernate on acute lesions in rats. Folia Pharmacol Jpn 1985;85:443-8.

Takeuchi K, Okabe S. Importance of pepsin and stomach distension in morphological alterations of stress-induced gastric lesions in pylorus ligated rats. Dig Dis Sci 1988;33:52-9.

Szabo S, Hollander D. Pathways of gastrointestinal protection and repair: mechanisms of action of sucralfate. Am J Med 1985;86:23-31.

Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with folin phenol reagent. J Biol Chem 1951;193:265-75.

Ellman GL. Tissue sulfhydryl groups. Arch Biochem Biophys 1959;82:70-7.

Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 1979;95:351-8.

Warren L. The thiobarbituric acid assay of sialic acids. J Biol Chem 1959;234:1971-5.

Winzler RJ. Determination of serum glycoproteins. In: Methods of Biochemical Analysis. Interscience Publisher: New York; 1955. p. 279-311.

Habig WH, Pabst MJ, Jakoby WB. Glutathione S-transferases. The first enzymatic step in mercapturic acid formation. J Biol Chem 1974;249:7130-9.

Paglia DE, Valentine WN. Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Clin Med 1967;70:158-69.

Takahara S, Hamilton HB, Neel JV, Kobara TY, Ogura Y, Nishimura ET. Hypocatalasemia: a new genetic carrier state. J Clin Invest 1960;39:610-9.

Misra HP, Fridovich I. The role of superoxide anion in the autoxidation of epinephrine and a simple assay for superoxide dismutase. J Biol Chem 1972;247:3170-5.

Oluwabunmi IJ, Abiola T. Gastroprotective effect of methanolic extract of Gomphrena celosioides on indomethacin induced gastric ulcer in Wistar albino rats. Int J Appl Basic Med Res 2015;5:41-5.

Mahendran P, Sabitha KE, Devi CS. Prevention of HCl-ethanol induced gastric mucosal injury in rats by Garcinia cambogia extract and its possible mechanism of action. Indian J Exp Biol 2002;40:58-62.

Venkataranganna MV, Gopumadhavan S, Sundaram R, Mitra SK. Evaluation of possible mechanism of anti-ulcerogenic activity of UL-409, a herbal preparation. J Ethnopharmacol 1998;63:187-92.

Jainu M, Vijai Mohan K, Shyamala Devi CS. Gastroprotective effect of Cissus quadrangularis extract in rats with experimentally induced ulcer. Indian J Med Res 2006;123:799-806.

Ganesan B, Yathavamoorthi R, Farvin KHS, Anandan R. Supplementation of Betaine attenuates HCl-ethanol induced gastric ulcer in rats. Int J Bio Chem 2010;4:79-89.

Sudheer KM, Sridhar RB, Kiran BS, Bhilegaonkar PM, Shirwaikar A, Unnikrishnan MK. Antiinflammatory and antiulcer activities of phytic acid in rats. Indian J Exp Biol 2004;42:179-85.

Deoda RS, Kumar D, Bhujbal SS. Gastroprotective effect of Rubia cordifolia Linn. on Aspirin plus pylorus ligated ulcer. BMC Complem Altern Med 2011;8(1):1.

Abraham G, Vidhu A. A preliminary examination of the phytochemical profile of Azolla microphylla with respect to seasons. Asian Pac J Trop Biomed 2012;S1392-S5.

Chowdhury R, Selvaraj K, Bhattacharjee C. Isolation and structural elucidation of flavonoids from aquatic fern Azolla microphylla and evaluation of free radical scavenging activity. Int J Pharm Pharm Sci 2013;5:743-9.

Pandian RS, Anuradha CV, Viswanathan P. Gastroprotective effect of fenugreek seeds (Trigonella foenum-graecum) on experimental gastric ulcer in rats. J Ethnopharmacol 2002;81:393-7.



How to Cite

Bhaskaran, S. K., A. Rangasamy, S. Sundaram, V. K. Gopalakrishnan, and P. Kannappan. “EFFECT OF AQUEOUS EXTRACT OF AZOLLA FILICULOIDES IN GASTRIC MUCOSA OF ULCERATED RATS”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 7, no. 7, July 2015, pp. 355-8, https://www.innovareacademics.in/journals/index.php/ijpps/article/view/6529.



Original Article(s)