PROBING THE BROAD-SPECTRUM THERAPEUTIC POTENTIAL OF AIP II MIMICS TO COMBAT LYSOZYME MEDIATED STAPHYLOCOCCAL INVASION ON CONTACT LENS
Keywords:
Staphylococcus aureus, Bacterial keratitis, AIP II mimics, Lysozyme, Contact LensAbstract
Objective: Molecular recognition of AIP II mimics as a global inhibitor against the AgrC variants and to undertake a real-time clinical applications to treat the lysozyme mediated (tear protein) S. aureus adherence on contact lens.
Methods: Structure activity relationship of the mimic peptides against the receptor AgrC variants were studied to score the global inhibitor. Further, the activity of the mimics as inhibitors was validated through in vitro and in vivo analysis.
Results: Inhibition of agr expression of interstrains by the mimic compounds gained insight to recognize a global inhibitorâ€. Further, the in vitro data were designed in such a way to provide a natural eye environment (artificial tears) to see the effect of mAIP IIa (IC50) showed a greater significance of eradicating the clinical isolate, S. aureus biofilm and various other secreted toxins.
Conclusion: The mimic peptide (mAIPII a) revealed to be a potential mimic of AIPII to show a broad range inhibition of all AgrC variants without any cytotoxic effects.
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