SIMULTANEOUS ESTIMATION OF TELMISARTAN AND HYDROCHLOROTHIAZIDE BY DERIVATIVE SPECTROSCOPY
Keywords:
Spectroscopy, Telmisartan, Hydrochlorothiazide, Derivative spectrophotometricAbstract
Objective: The objective of this investigation is to present the simple and sensitive method for the analysis of Telmisartan and Hydrochlorothiazide and apply the proposed method for the analysis of said drugs in pharmaceutical formulations.
Methods: Telmisartan and Hydrochlorothiazide are used in combination for treatment of hypertension. The present work deals with simple derivative spectrophotometric method development for simultaneous estimation of Telmisartan and Hydrochlorothiazide in two component tablet formulation.
Results: The method employed first order derivative spectroscopy for determination of ʎmax by taking 10 μg/ml each of Telmisartan and Hydrochlorothiazide were scanned in 200-400 nm range and ʎmax were observed 291 nm for Telmisartan and Hydrochlorothiazide showed zero crossing point and 251 nm for Hydrochlorothiazide and Telmisartan showed zero crossing point in first order derivative spectroscopy.
Conclusion: For this method, linearity was observed in 2-40 μg/ml for Telmisartan and 2-20 μg/ml for Hydrochlorothiazide. The recovery study confirmed the accuracy of the proposed method and low values of standard deviation confirmed precision of the used method. The method was validated as per ICH guidelines.
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References
International Conference on Harmonization, ICH Q 2B (R1). Validation of Analytical Procedures: Methodology; 1996.
Beckett AH, Stenlake JB. Practical Pharmaceutical Chemistry. 2nd ed. New Delhi: CBS Publishers and Distributors; 1988.
Chatwal GR, Anand SK. Instrumental Methods of Chemical Analysis. 4th ed. Mumbai: Himalaya Publishing House; 1994.
Willard. Instrumental Methods of Analysis. 7th ed. New Delhi: CBS Publishers and Distributors; 1986.
Sharma BK. Instrumental Methods of Chemical Analysis. 20th ed. Meerut: Krishna Prakashan Media; 2001.
Sethi PD. Quantitative analysis of drugs in pharmaceutical formulations. 3rd ed. New Delhi: CBS Publishers and Distributors; 2001.
Bankey S, Tapadiya GG, Saboo SS, Bindaiya S, Jain Deepti, Khadbadi SS. Simultaneous determination of ramipril, Hydrochlorothizide and telmisartan by Spectrophotometry. Int J Chem Tech Res; 2009;1(2):183-8.
Patil Kiran R, Rane Vipul P, Sangshetti Jaiprakash N, Shinde Devanand B. A Stability-Indicating LC method for the simultaneous determination of telmisartan and ramipril in dosage form. Chromatographia 2008;67:575-82.
Mohitea Popat B, Pandharea Ramdas B, H Bhaskarb Vaidhun. Simultaneous Estimation of ramipril and telmisartan in tablet dosage form by spectrophotometry Eurasian. J Anal Chem 2010;5(1):89-94.
Kayal SD, Khan F. A Method development and validation for the simultaneous determination of amlodipine besylate and telmisartan in tablet dosage form by Rp-Hplc. Int J Pharm Res Dev 2011;3:5.
Prasad CVN, Santhosh Kumari, Ramulu Sree. Simultaneous determination of telmisartan, amlodipine besylate and hydrochlorothiazide in a combined poly pill dosage form by stability-indicating high performance liquid chromatography. Int J Res Pharm Chem 2011;1(3);69-84.
www.drugbank.ca/drugs.