• Krishna Naragani
  • Ushakiranmayi Mangamuri
  • Vijayalakshmi Muvva Department of Botany and Microbiology, Acharya Nagarjuna University, Guntur 522510, Andhra Pradesh, India
  • Sudhakar Poda
  • Rajesh Kumar Munaganti


Objectives: The aim of the present study was to isolate, characterize and evaluate the activity of compounds produced by Streptomyces cheonanensis VUK-A.

Methods: Chemical examination of the secondary metabolites of the strain Streptomyces cheonanensis VUK-A has led to the segregation of one bioactive compound (1) and a partially purified fraction (2). The strain was isolated from the sediment samples of mangrove ecosystem of Coringa, south coastal Andhra Pradesh, India. The chemical structure of the active compound 1 was established on the basis of spectroscopic analysis including 1H NMR, 13C NMR spectroscopy, FTIR and EIMS. The partially purified sub-fraction (2) subjected to Gas Chromatography-Mass spectroscopy. The antimicrobial activity of the bioactive compounds produced by the strain was expressed in terms of minimum inhibitory concentration.

Results: The compound 1 was isolated from the fermentation broth was characterized as benzoic acid (1) based on spectroscopic analysis. The partially purified sub-fraction (2) subjected to Gas Chromatography-Mass spectroscopy contained nine analogues: 1-tetradecene, tetradecane, 1-hexadecene, hexadecane, 5-octadecene, octadecane, 5-eicosene, 1-nonadecene and cyclo tetracosane. The compounds recorded moderate to significant antimicrobial activity against medicinally and agriculturally important bacteria and fungi. This is the first report of six partially purified compounds 1-tetradecene, tetradecane, hexadecane, octadecane, 5-eicosene and cyclo tetracosane from the genus Streptomyces.

Conclusion: The results of the present study showed that the metabolites of Streptomyces cheonanensis VUK-A exhibited antibacterial and antifungal activities. The study also supports that Coringa, a promising mangrove ecosystem remained to be explored for new bioactive compounds.

Keywords: Streptomyces cheonanensis, Mangrove Ecosystem, Natural Products, Antimicrobial activity.


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Ganesan G, Velayudhan SS, Solomon RDJ. Antimicrobial potential of phylogenetically unique actinomycete, Streptomyces sp. JRG-04 from marine origin. Biologicals 2014;42:205-311.

Radhika S, Bharathi S, Radhakrishnan M, Balagurunathan R. Bioprospecting of fresh water actinobacteria: isolation, antagonistic potential and characterization of selected isolates. J Pharm Res 2011;4:2584-6.

Arijit D, Sourav B, Abuelgassim Y, Hassan M, Subbaramiah SR. In vitro antimicrobial activity and characterization of mangrove isolates of Streptomycetes effective against bacteria and fungi of nosocomial origin. Braz Arch Biol Technol 2014;57:349-56.

Giri C, Ochieng E, Tieszen LL, Zhu Z, Singh A, Lovel T, et al. Status and distribution of mangrove forests of the world using earth observation satellite data. Global Ecology Biogeograph 2011;20:154-9.

Naikpatil SV, Rathod JL. Selective isolation and antimicrobial activity of rare actinomycetes from mangrove sediment of Karwar. J Ecobiotechnol 2011;3:48-53.

Singh MP, Petersen PJ, Weiss WJ, Janso JE, Luckman SW, Lenoy EB, et al. Mannopeptimycins, new cyclic glycopeptide antibiotics produced by Streptomyces hygroscopicus LL-AC98: antibacterial and mechanistic activities. Antimicrob Agents Chemother 2003;47:62-9.

Bentley SD, Chater KF, Cerdeño-Tárraga AM, Challis GL, Thomson NR, James KD, et al. Complete genome sequence of the model actinomycete Streptomyces coelicolor A3. Nature 2002;41:141-7.

Usha R, Ananthaselvi P, Venil CK, Palaniswamy M. Antimicrobial and anti-angiogenesis activity of Streptomyces pravulus KUAP1067 from mangrove soil. Eurasian J Biosci 2010;2:77-83.

Zin NM, Sarmin NIM, Ghadin N, Basri DF, Sidik NM, Hess WM, et al. Bioactive endophytic Streptomycetes from the malay peninsula. FEMS Microbiol Lett 2007;274:83-8.

Berdy J. Bioactive microbial metabolites; a personal view. J Antibiot 2005;58:1-26.

Goshi K, Uchida T, Lezhava A, Yamasaki M, Hiratsu K, Shinkawa K, et al. Cloning and analysis of the telomere and terminal inverted repeat of the linear chromosome of Streptomyces griseus. J Bacteriol 2005;184:3411-5.

Bibb MJ. Regulation of secondary metabolism in Streptomyces. Curr Opin Microbiol 2005;8:208-15.

Huo H, Hai-Peng L, Qingyi X, Lei L, Xin-Qiang X, Kui H. Streptomyces qinglanensis sp. nov., isolated from mangrove sediment. Int J Syst Evol Microbiol 2012;62:596-600.

Ushakiranmayi M, Sudhakar P, Krishna N, Vijayalakshmi M. Influence of cultural conditions for improved production of bioactive metabolites by Streptomyces cheonanensis VUK-A isolated from coringa mangrove ecosystem. Curr Tren Biotechnol Pharm 2012;6:99-111.

Boussaada O, Ammar A, Saidana D, Chriaa J, Chraif I, Daami M, et al. Chemical composition and antimicrobial activity of volatile components from capitula and aerial parts of Rhaponticum acaule DC growing wild in Tunisia. Microbiol Res 2008;163:87-95.

Cappuccino JG, Sherman N. Editors. 5th ed. In: Microbiology: A laboratory manual, Benjamin, Harlow; 2002. p. 263-4.

Beuchat LR. Comparison of anti-vibrio activities of potassium sorbate, sodium benzoate, glycerol and sucrose esters of fatty acids. Appl Environ Microbiol 1980;39:1178-82.

Yousef AE, El-shenawy MA, Marth EH. Inactivation and injury of Listeria monocytogenes in minimal medium as affected by benzoic acid and incubation temperature. J Food Sci 2008;54:650-52.

Rahn O, Conn JC. Effect of increase in acidity on antiseptic efficiency. J Ind Eng Chem 1944;36:185-7.

Rusul G, Marth EH. Food additives and plant components control growth and aflatoxin production by toxigenic Aspergilli: a review. Mycopathlogia 1988;101:13-23.

Pelayo US. In vitro effectiveness of salicylic, benzoic and boric acid and diiodine thymol solutions on various strains of dermatophytes. Rev Cubana Med Trop 1979;31:121-6.

Smaoui S, Mathieu F, Elleuch L, Coppel Y, Merlina G, Karray-Rebai I, et al. Taxonomy, purification and chemical characterization of four bioactive compounds from new Streptomyces sp. TN256 strain. World J Microbiol Biotechnol 2011;28:793-804.

El-Sakhawy FS, El-Tantawy ME, Ross SA, El-Sohly MA. Composition and antimicrobial activity of the essential oil of Murraya exotica. Flavour Fragrance J 1998;13:59-62.

Lay-Jing S, Hooi-Kheng B, Pazilah I, Amirin S, Mohd ZA. Antimicrobial activity of Gynura segetum's leaf extracts and its active fractions. Int J Gen Trad Med 2012;2:1-5.

Elleuch L, Shaaban KA, Abdel-Aziz MS, Chakchouk A, Nagia MMS, Mellouli L, et al. Cyclic lipopeptides and other bioactive secondary metabolites from a new terrestrial Streptomyces sp. TN272. Afr J Microbiol Res 2012;6:2202-10.



How to Cite

Naragani, K., U. Mangamuri, V. Muvva, S. Poda, and R. K. Munaganti. “ANTIMICROBIAL POTENTIAL OF STREPTOMYCES CHEONANENSIS VUK-A FROM MANGROVE ORIGIN”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 8, no. 3, Mar. 2016, pp. 53-57,



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