MOLECULAR ASSESSMENT OF GENETIC DIVERSITY IN INDIAN ACCESSIONS OF ALOE VERA USING SSR MARKER
Keywords:Aloe vera, Agro-climatic zones, SSR, Genetic diversity, Polymorphism
Objective: In this study Aloe vera (L.) Burm. f. collected from 12 states covering all the different agro-climatic zones of India were investigated for its genetic diversity analysis by using SSR marker assay.
Methods: Total genomic DNA was isolated from young leaf samples using CTAB method. Twenty primers were selected which were used for Asparagus officinalis L a related species of A. vera and others were developed from available Aloe vera plant sequences with the help of primer 3 software. Similarity matrices and dendrogram were constructed by using NTSys software to show a phenetic representation of the genetic relationship. Polymorphic Information Content (PIC), the effective multiplex ratio (EMR) and Marker Index (MI) were calculated for the assessment of genetic diversity.
Results: The neighbor-joining tree based on all SSR fragments of twelve Aloe vera germplasm accessions grouped into three major clusters. The similarity value ranged from 46 % to 100 %. The highest 100 % similarity was noted between Haryana and Uttar Pradesh accessions followed by 93% similarity between Haryana and Punjab accessions with Rajasthan. Minimum similarity was noted between Gujarat and Kerala accessions.
Conclusion: This study revealed the rich genetic diversity among Aloe vera accessions from different agro-climatic zones of India. It is also concluded that SSR marker analysis can be a useful tool for the assessment of genetic diversity of the medicinal plants.
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