MOLECULAR ASSESSMENT OF GENETIC DIVERSITY IN INDIAN ACCESSIONS OF ALOE VERA USING SSR MARKER

Authors

  • Sandeep Kumar M. D. University
  • Manila Yadav M. D. University
  • Amita Yadav M. D. University
  • Jaya Parkash Yadav Department of Genetics, M. D. University, Rohtak 124001, Haryana, India

Keywords:

Aloe vera, Agro-climatic zones, SSR, Genetic diversity, Polymorphism

Abstract

Objective: In this study Aloe vera (L.) Burm. f. collected from 12 states covering all the different agro-climatic zones of India were investigated for its genetic diversity analysis by using SSR marker assay.

Methods: Total genomic DNA was isolated from young leaf samples using CTAB method. Twenty primers were selected which were used for Asparagus officinalis L a related species of A. vera and others were developed from available Aloe vera plant sequences with the help of primer 3 software. Similarity matrices and dendrogram were constructed by using NTSys software to show a phenetic representation of the genetic relationship. Polymorphic Information Content (PIC), the effective multiplex ratio (EMR) and Marker Index (MI) were calculated for the assessment of genetic diversity.

Results: The neighbor-joining tree based on all SSR fragments of twelve Aloe vera germplasm accessions grouped into three major clusters. The similarity value ranged from 46 % to 100 %. The highest 100 % similarity was noted between Haryana and Uttar Pradesh accessions followed by 93% similarity between Haryana and Punjab accessions with Rajasthan. Minimum similarity was noted between Gujarat and Kerala accessions.

Conclusion: This study revealed the rich genetic diversity among Aloe vera accessions from different agro-climatic zones of India. It is also concluded that SSR marker analysis can be a useful tool for the assessment of genetic diversity of the medicinal plants.

 

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Author Biography

Jaya Parkash Yadav, Department of Genetics, M. D. University, Rohtak 124001, Haryana, India

Sandeep Kumar, Research Scholar

Department of Genetics, M. D. University, Rohtak-124001, Haryana,India

Manila Yadav, Research Scholar

Department of Genetics, M. D. University, Rohtak-124001, Haryana, India

Amita Yadav, Research Scholar

Department of Genetics, M. D. University, Rohtak-124001, Haryana, India

Jaya Parkash Yadav, Professor

Department of Genetics, M. D. University, Rohtak-124001, Haryana, India

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Published

01-12-2015

How to Cite

Kumar, S., M. Yadav, A. Yadav, and J. P. Yadav. “MOLECULAR ASSESSMENT OF GENETIC DIVERSITY IN INDIAN ACCESSIONS OF ALOE VERA USING SSR MARKER”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 7, no. 12, Dec. 2015, pp. 149-54, https://www.innovareacademics.in/journals/index.php/ijpps/article/view/8664.

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