FORMULATION AND ASSESSMENT OF A HERBAL HAIR CREAM AGAINST CERTAIN DERMATOPHYTES
Objective: Developing an herbal antifungal formulation containing eruca and garlic oils against highly resistant dermatophytes (Malassezia fufur AUMC No. 5173, Microsporum canis bodin AUMC No. 5490 and Trichophyton mentagrophytes AUMC No. 5501. 5501) and assessment of garlic oil thiosulfonates during the ex vivo percutaneous permeation through albino rat skin.
Methods: Assay of antifungal activity was performed by filter paper disc method and agar well diffusion method. The components of volatile constituents and fixed oil of eruca seeds were studied using GC/MS. Thiosulfinates in garlic oil were analyzed by HPLC/UV. Both oils were incorporated into hair cream using span 60 and brij 58 at three different concentrations (2, 4 and 6% w/w) and alliin, was ex vivo evaluated using albino rat skin mounted on Franz diffusion cells.
Results: The two oils have a synergistic effect on the first and additive effect on the second and the third fungi. The main constituents in eruca are 4-(methyl thio) butyl isothiocyanate (82%) for volatile constituents and erucic acid (40%) for the fixed one. The highest flux for alliin (0.337Â±0.0015 mg/cm2/hr) was obtained at a 4% surfactant concentration.
Conclusion: Combination of oils has a high activity on the selected dermatophytes. Formulation of an herbal hair cream using span 60 and Brij 58 with a concentration 4% gives the highest permeation rate for alliin in garlic oil.
Keywords: Eruca, Garlic, Dermatophytes, Quantitative determination and Ex-vivo permeation
Khoobchandani M, Ojeswi BK, Ganesh N, Srivastava MM, Gabbanini S, Matera R, et al. Antimicrobial properties and analytical profile of traditional Eruca sativa seed oil: comparison with various aerial and root plant extracts. Food Chem 2010;120:217-24.
Savoia D. Plant-derived antimicrobial compounds: alternatives to antibiotics. Future Microbiol 2012;7:979-90.
Simoes M, Bennett RN, Rosa EAS. Understanding antimicrobial activities of phytochemicals against multidrug resistant bacteria and biofilms. National Public Radio 2009;26:746-57.
Tra FHB, KonÃ© MW, KouamÃ© NF. Antifungal activity of erigeron floribundus (Asteraceae) from cÃ´te dâ€™Ivoire, West Africa. Trop J Pharm Res 2008;7:975-9.
Dias MFRG, Quaresma-Santos MVP, Bernardes-Filho F, Amorim AGF, Schechtman RC, Azulay DR, et al. Update on therapy for superficial mycoses: review article part I. An Bras Dermatol 2013;88:764â€“74.
The Center for Food Security and Public Health. Dermatophytosis. Iowa state university, College of Veterinary Medicine, (Ames, Iowa); 2013. Available from: www.cfsph.iastate.edu. [Last accessed on 20 Aug 2015].
Shenefelt PD. Herbal treatment for dermatological disorders. In: Benzie IFF, Wachtel-Galor S. Editors. Herbal Medicine: Biomolecular and Clinical Aspects. 2nd ed. Boca Raton (FL): CRC Press; 2011. p. 383.
Sumit K, Vivek S, Sujata S, Ashish B, Rapid I. Herbal cosmetics: used for skin and hair. Inventi Rapid: Cosmeceuticals; 2012.
Qi R, Wang Z. Pharmacological effects of garlic extract. Trends Pharmacol Sci 2003;24:62-3.
Juszkiewicz A, Zaborska A, ÅaptaÅ› A, Olech Z. A study of the inhibition of jack bean urease by garlic extract. Food Chem 2004;85:553â€“8.
Hiyasat B, Sabha D, Grotzinger K, Kempfert J, Rauwald JW, Mohr FW, et al. Antiplatelet activity of Allium ursinum and Allium sativum. Pharmacology 2009;83:197-4.
Stool A, Seebeck E. Allium compounds. I. Alliine, the true mother compound of garlic oil. Helv Chim Acta 1949;31:197-9.
Ledezma E, De Sousa L, Jorquera A. Efficacy of ajoene, an organosulphur derived from garlic, in the short-term therapy of tinea pedis. Mycoses 1996;39:393-5.
Bianco VV. Rocket, an ancient underutilized vegetable crop and its potential. In: Padulosi S. Editors. Rocket Genetic Resources Network. Report of the First Meeting, Lisbon, Portugal; 1994.
Nuez F, HernÃ¡ndez Bermejo JE. Neglected horticultural crops. In: HernÃ¡ndo Bermejo JE, LeÃ³n J. Editors. Neglected Crops: 1492 from a different perspective. Plant Production and Protection Series 26. FAO, Rome, Italy; 1994. p. 303-32.
Filocamo A, Paterniti Mastrazzo G, Maimone P, Buongiorno L, Catania S, De Pasquale R, et al. Eruca sativa Mill: phytochemical profile and antimicrobial properties of rocket leafy salads. Planta Med 2010;76:WSI_1.
Al Khalil S. A survey of plants used in jordanian traditional medicine. Pharm Biol 1995;33:317-23.
Murray PR, Baron EJ, Pfaller MA, Tenover FC, Yolke RH. Manual of clinical microbiology. 6th ed. ASM, Washington, DC; 1995.
Vogel AI. Practical organic chemistry. 3rd ed. London: Longmans, Green; 1961.
Johnson AR, Davenport JP. Biochemistry and methodology of lipids. New York: Wiley; 1971. p. 35.
Dhawan B, Aggarwal G, Harikumar SL. Enhanced transdermal permeability of piroxicam through novel nanoemulgel formulation. Int J Pharm Investig 2014;4:65-76.
Gabbanini S, Lucchia E, Carli M, Berlini E, Minghetti A, Valgimiglib L, et al. In vitro evaluation of the permeation through the reconstructed human epidermis of essentials oils from cosmetic formulations. J Pharm Biomed Anal 2009;50:370-6.
Patel RP, Gaiakwad DR, Patel NA. Formulation, optimization, and evaluation of a transdermal patch of heparin sodium. Drug Discoveries Ther 2014;8:185-93.
El Meshad AN, Tadros MI. Transdermal delivery of an anti-cancer drug via W/O emulsions based on alkyl poly glycosides and lecithin: design, characterization, and in vivo evaluation of the possible irritation potential in rats. AAPS PharmSciTech 2011;12:1-9.
AraÃºjo CR, Miranda KC, Fernandes OFL, Soares AJ, Silva MRR. In vitro susceptibility testing of dermatophytes isolated in goiania, brazil, against five antifungal agents by broth microdilution method. Rev Inst Med Trop 2009;51:9-12.
Aala F, Yusuf UK, Jamal F, Rezaie R. Antimicrobial effects of allicin and ketoconazole on trichophytonrubrum under in vitro condition. Braz J Microbiol 2012;43:2.
Kim SJ, Ishii G. Glucosinolate profiles in the seeds, leaves and roots of rocket salad (Eruca sativa Mill.) and anti-oxidative activities of intact plant powder and purified 4-methoxyglucobrassicin. J Soil Sci Plant Nutr 2006;52:394-400.
Miyazawa M, Maehara T, Kurose K. Composition of the essential oil from the leaves of Eruca sativa. Flavour Fragrance J 2001;17:187â€“90.
PÃ¢rvu M, PÃ¢rvu AE, Vlase L, Casian OR, PÃ¢rvu O, PuÅŸcaÅŸ M, et al. Allicin and alliin content and antifungal activity of Allium senescens L. ssp. Montanum (F. W. Schmidt) Holub ethanol extract. J Med Plants Res 2011;5:544-9.
Arnault JP, Christide`s, Mandon N, Haffner T, Kahane R, Auger J, et al. High-performance ion-pair chromatography method for simultaneous analysis of alliin, deoxyalliin, allicin and dipeptide precursors in garlic products using multiple mass spectrometry and UV detection. J Chromatogr A 2003;991:69â€“75.
Barel AO, Paye M, Maibach HI. Handbook of cosmetic science and technology. 4th ed. Taylor and Francis group (NY); CRC Press; 2014. p. 273.
Babu RJ, Kikwai L, Jaiani LT, Kanikkannan N, Armstrong CA, Ansel JC, et al. Percutaneous absorption and anti-inflammatory effect of a substance P receptor antagonist: spantide II. Pharm Res 2004;21:108-13.
Williams AC, Barry BW. Penetration enhancers. Adv Drug Delivery Rev 2004;56:603-18.
Lane ME. Skin penetration enhancers. Int J Pharm 2013; 447:12-21.
Ibrahim SA, Kevin Li S. Efficiency of fatty acids as chemical penetration enhancers: mechanisms and structure enhancement relationship. Pharm Res 2010;27:115-25.
Amina S, Mirb SR, Kohlia K, Alib B, Ali M. A study of the chemical composition of black cumin oil and its effect on penetration enhancement from transdermal formulations. Nat Prod Res 2010;24:1151-7.
Kamboj S, Saini V, Bala S. Formulation and characterization of drug loaded nonionic surfactant vesicles (Niosomes) for oral bioavailability enhancement. Sci World J 2014. Doi.Org/10.1155/2014/959741. [Article in Press]
Butani D, Yewale C, Misra K. Amphotericin B topical microemulsion: formulation, characterization, and evaluation. Colloids Surf B 2014;116:351-8.
Sakulku U, Nuchuchua O, Uawongyart N, Puttipipatkhachorn S, Soottitantawat A, Ruktanonchai U, et al. Characterization and mosquito repellent activity of citronella oil nanoemulsion. Int J Pharm 2009;372:105-11.
Shokri J, Nokhodchi A, Dashbolaghi A, Hassan-Zadeh D, Ghafourian T, Jalali MB, et al. The effect of surfactants on the skin penetration of diazepam. Int J Pharm 2001;228:99â€“107.
Javadzadeh Y, Hamishehkar H. Enhancing percutaneous delivery of methotrexate using different types of surfactants. Colloids Surf B 2011;82:422-6.