• Sneha Sunil Sawant
  • Priyanka Murkute
  • A. M. Bhagwat
  • Varsha Kelkar Mane Department of Biotechnology, University of Mumbai, Kalina, Santacruz, Mumbai, Maharashtra, India, 400098


Objective: At low concentrations copper is an essential micronutrient for algal growth wherein it plays a vital role as an enzyme cofactor for photosynthetic processes but at high concentrations it functions as a toxic heavy metal. Copper exposure to microalga increases the activity of antioxidative enzymes–catalase, superoxide dismutase, peroxidase along with the induction of chlorosis at higher concentrations. The objective of the present study is to determine the effect of suboptimal concentration of copper on the generation time as well as the antioxidant potential of a novel strain-Chlorella emersonii KJ725233.

Methods: The growth of the microalga at the different copper concentrations was monitored by measuring the absorbance at 684 nm. Once the suboptimum copper concentration for the growth of the microalga was determined, the methanolic extracts were evaluated for Total Phenolic Content, Total Flavonoid Contents as well as the antioxidant potential by employing standard methods such as phospho- molybdenum method for Total Antioxidant Capacity and Ferric Reducing Antioxidant Potential.

Results: Results indicated that 0.1 µmol of copper stimulated the growth of Chlorella emersonii KJ725233, which was evident from the reduced generation time of the microalga as compared to that of the control and other copper concentrations. The study also proved a 45.69% increase in the antioxidant activity of the microalga on exposure to 0.1 µmol of copper.

Conclusion: The present study indicated copper at 0.1 µmol concentration not only acts as a micronutrient but also levies stress resulting in increased antioxidant activity of this novel isolate Chlorella emersonii KJ725233.

Keywords: Chlorella emersonii KJ725233, Copper, Generation time, Antioxidant


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Ernani P, Teresa CS, Maria ASL, Oswaldo KO, David M, Pio C. Heavy metal–induced oxidative stress in algae. J Phycol 2003;39:1008-18.

Gintare S. Copper-induced physiological changes and oxidative damage in lichen Ramalina farinacea. Biologija 2014;60:196-201.

Devi YM, Mehta SK. Changes in antioxidative enzymes of cyanobacterium Nostoc muscorum under copper (Cu2+) stress. Sci Vision 2014;14:207–14.

Murugan K, Harish SR. Antioxidant modulation in response to heavy metal-induced oxidative stress in Cladophora glomerata. Indian J Exp Biol 2007;45:980-3.

Pantola RC, Shekhawat GS. Copper-induced antioxidative enzyme indices in leaves of Brassica juncea seedlings. J Pharm Biomed Sci 2012;15:1-6.

Chi YL, Yong HY, Sidhartan M, Chae WM, In CB, Jong MK, et al. Effects of copper (I) oxide on growth and biochemical compositions of two marine microalgae. J Environ Biol 2006;27:461–6.

Rashad K, Yassin EA, Asmaa H. Effect of copper on growth, bioactive metabolites, antioxidant enzymes and photosynthesis-related gene transcription in Chlorella vulgaris. World J Biol Sci 2014;2:34-43.

Sawant SS, Joshi AJ, Bhagwat A, Mane V. Tapping the antioxidant potential of a novel isolate–Chlorella emersonii. World J Pharm Res 2014;3:726-39.

Ramasamy S, Sanniyasi E, Sundaram S. Fatty acid methyl ester analysis of potent microalgae Scenedesmus dimorphus (Turpin) kutzing and Chlorococcum infusionum (Schrank) meneghini isolated from effluents of Neyveni thermal power station expansion 1. J Algal Biomass Utilization 2012;3:12-20.

Wu LC, Ho JA, Shieh MC, Lu IW. Antioxidant and antiproliferative activities of Spirulina and Chlorella water extracts. J Agric Food Chem 2005;53:4207-12.

Edewor–Kuponiyi TI. Spectroscopic determination of total phenolic and total flavonoid contents, the antioxidant activity of the leaves of Persea Americana. Int J Pharm Pharm Sci 2013;5:598-603.

Hemalatha A, Girija K, Parthiban C, Saranya, Anantharaman P. Antioxidant properties and total phenolic content of a marine diatom, Navicula clavata and green microalgae, Chlorella marina and Dunaliella salina. Adv Appl Sci Res 2013;4:151-7.

Li Y, Mu J, Chen D, Han F, Xu H, Kong F, et al. Production of biomass and lipid by microalgae Chlorella protothecoides with heterotrophic–Cu (II) stressed (HCuS) coupling cultivation. Bioresour Technol 2013;148:283-92.

Manivannan K, Ananthraman P, Balasubramanian T. Evaluation of antioxidant properties of marine microalga Chlorella marina. Asian Pac J Trop Biomed 2012;2:S342-6.

Saranya C, Hemalatha A, Parthiban C, Ananthraman P. Evaluation of antioxidant properties, total phenolic content and carotenoid content of Chaetoceros calcitrans, Chlorella salina and Isochyrsis galbana. Int J Curr Microbiol Appl Sci 2014;3:365-77.



How to Cite

Sawant, S. S., P. Murkute, A. M. Bhagwat, and V. K. Mane. “EFFECT OF COPPER ON THE GENERATION TIME AND ANTIOXIDANT POTENTIAL OF A NOVEL ISOLATE OF CHLORELLA EMERSONII KJ725233”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 8, no. 3, Mar. 2016, pp. 385-7, https://www.innovareacademics.in/journals/index.php/ijpps/article/view/9598.



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