• Ridhima Wadhwa
  • Yash Sharma
  • Renuka Pandey Upadhyay
  • Kumud Bala Centre of Biotechnology, Amity Institute of Biotechnology, Amity University Noida, U.P, India


Nosocomial infection, Gram-negative Bacteria, Vitek-2, Drug Resistance


Objective: The purpose of the present study was to identify the Non-Fermenting Gram Negative Bacteria from the tertiary care hospital.

Methods: The conventional method was used i.e. Antibiotic susceptibility test which was performed by Kirby–Bauer disc diffusion method and analysis of microbes was done by Vitek-2.

Results: 172 samples were identified as Non-Fermenting Gram-Negative Bacilli from 1526 respiratory samples. Acinetobacter baumanii was the predominant isolate accounting 103 (59.88%) specimens followed by Pseudomonas aeruginosa 33.13%. Other isolates were Stenotrophomonas maltophilia 5.23% and Burkholderi acepacia1.74%. From the 172 samples, Endotracheal Aspirate was found to be the most infected amongst all the other samples. Acinetobacter baumanii and Pseudomonas aeruginosa were the most common NFGNB isolated in our study from patients of Respiratory Tract Infections.

Conclusion: Both Acinetobacter baumanii and Pseudomonas aeruginosa showed good sensitivity to colistin, amikacin, cefoperazone while in most cases carbapenam is found to be highly resistant. Colistin along with amikacin and cefoperazone should be used against this pathogen for treatment.

Keywords: Nosocomial infection, Gram negative bacilli, Tertiary care hospital, Lower respiratory tract infection, Non-fermenting bacilli


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How to Cite

Wadhwa, R., Y. Sharma, R. P. Upadhyay, and K. Bala. “NOSOCOMIAL INFECTION BY NON-FERMENTING GRAM NEGATIVE BACILLI IN TERTIARY CARE HOSPITAL: SCREENING AND CURE”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 8, no. 3, Mar. 2016, pp. 274-7,



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