@article{Netala_Kotakadi_Ghosh_Bobbu_Nagam_K. K._Tartte_2014, title={BIOFABRICATION OF SILVER NANOPARTICLES USING AQUEOUS LEAF EXTRACT OF MELIA DUBIA, CHARACTERIZATION AND ANTIFUNGAL ACTIVITY}, volume={6}, url={https://journals.innovareacademics.in/index.php/ijpps/article/view/2710}, abstractNote={<p><strong>Objective: </strong>To investigate the bio-fabrication of silver nanoparticles (AgNPs) using aqueous leaf extract of <em>Melia dubia</em> (ALM) and test the antifungal activity of AgNPs against pathogenic fungi <em>Aspergillus niger </em>and <em>Candida tropicalis.</em></p> <p><strong>Methods: </strong>10 ml of aqueous leaf extract of <em>Melia dubia </em>was added to 90 ml of 1 mM silver nitrate and incubated for 8h at room temperature. After incubation, the color change was observed from light yellow to dark brown. The synthesized AgNPs were characterized using UV-Vis spectroscopy, Fourier Transform Infra red spectroscopy (FTIR), Energy Dispersive X-ray Spectroscopy (EDX), Scanning Electron microscopy (SEM), X-ray diffraction analysis (XRD) and Atomic Force Microscopy (AFM). Antifungal activity against <em>Aspergillus niger </em>and <em>Candida tropicalis</em> was carried out by employing the disc diffusion method.</p> <p><strong>Results: </strong>UV-Vis spectra confirmed the synthesis of AgNPs by showing characteristic peak between 380-450 nm*. FTIR spectra showed the functional groups possibly involved in the synthesis of AgNPs. EDX confirms the presence of elemental silver at 3 Kev. SEM and AFM showed the synthesized AgNPs were spherical in shape with size ranging between 20-40 nm*. XRD analysis revealed the crystalline nature of AgNPs with face centred cubic (FCC) lattice. AgNPs was found to be very effective against the tested pathogenic fungi <em>A. niger</em> and <em>C. tropicalis </em>and formed the inhibition zones 13.0 and 14.5 mm respectively.</p> <p><strong>Conclusion: </strong>It is concluded that the bio-fabrication of AgNPs using aqueous leaf extract of <em>Melia dubia </em>was robust and rapid. The AgNPs were stable and proved to be excellent antifungal agents.</p>}, number={10}, journal={International Journal of Pharmacy and Pharmaceutical Sciences}, author={Netala, Vasudeva Reddy and Kotakadi, Venkata Subbaiah and Ghosh, Sukhendu Bikash and Bobbu, Pushpalatha and Nagam, Venkateswarlu and K. K., Sharma and Tartte, Vijaya}, year={2014}, month={Oct.}, pages={298–300} }