TY - JOUR AU - Narendhran, S. AU - Rajiv, P. AU - Vanathi, P. AU - Sivaraj, Rajeshwari PY - 2014/07/01 Y2 - 2024/03/29 TI - SPECTROSCOPIC ANALYSIS OF BIOACTIVE COMPOUNDS FROM STREPTOMYCES CAVOURESIS KUV39: EVALUATION OF ANTIOXIDANT AND CYTOTOXICITY ACTIVITY JF - International Journal of Pharmacy and Pharmaceutical Sciences JA - Int J Pharm Pharm Sci VL - 6 IS - 7 SE - Original Article(s) DO - UR - https://journals.innovareacademics.in/index.php/ijpps/article/view/2009 SP - 319-322 AB - <p><strong>Objective:</strong> To assess the antioxidant and anticancer activity of crude ethyl acetate extract from <em>Streptomyces cavouresis</em> KU-V39 isolated from vermicompost.</p><p><strong>Methods: </strong>To determine its antioxidant activity by total phenol and flavonoid content, Hydrogen peroxide radical scavenging activity and Ferrous reducing power assay. To determine <em>in vitro </em>cytotoxicity, various concentrations of extract was tested on HeLa cell line by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. Fourier transform infrared spectroscopy (FT-IR) and Gas chromatography–mass spectroscopy (GC-MS) have been carried out to investigate the bioactive compound of crude extract.</p><p><strong>Results: </strong>The Phenol and flavonoid content of crude extract was found to be 20.24 and 26.60 mg /g metabolite. Isolate KU-V39 exhibited H<sub>2</sub>O<sub>2</sub> scavenging activity at IC<sub>50 </sub>value is 42.35 ± 0.75 μg/ml. The ferrous reducing power assay has higher absorbance value which indicates the high antioxidant capacity of the extracts. The analysis of FT-IR spectroscopy indicate presents of Phenol, carboxylic acid, alkanes, nitro and aliphatic amines functional groups. The GC – MS analysis revealed that presence of Pentanoic acid, L-Proline, Lysine, Erucic acid, isosteviol, Pentadecanoic acid, Phthalic acid, Hexadecanoic acid, Octadecenoic acid, Dichloroacetic acid, Cyclohexanecarboxylic acid and Pyrrolo pyrazine derivative.</p><p><strong>Conclusion:</strong> This spectral study clearly proved that the vermicompost derived <em>Streptomyces</em> with extra cellular metabolism showed promising antioxidant and anticancer activity.</p> ER -