TY - JOUR AU - Ebrahimi, Lida AU - Nawaz, K. A. Ayub AU - Kiran, K. G. AU - Nisshanthini, S. Durairaju AU - Palaniswamy, Muthusamy AU - Angayarkanni, Jayaraman PY - 2016/02/01 Y2 - 2024/03/29 TI - PRODUCTION AND PURIFICATION OF ANGIOTENSIN-CONVERTING ENZYME INHIBITOR BY SELECTED BACTERIAL STRAIN FOR CANCER THERAPY JF - International Journal of Pharmacy and Pharmaceutical Sciences JA - Int J Pharm Pharm Sci VL - 8 IS - 2 SE - Original Article(s) DO - UR - https://journals.innovareacademics.in/index.php/ijpps/article/view/8389 SP - 214-220 AB - <p><strong>Objective: </strong>The present study was planned to explore safer, innovative and economic Angiotensin-converting enzyme inhibitors (ACEi) from beef extract by the action of a proteolytic <em>Micrococcus luteus</em>. Cytotoxicity of the stable peptide was predicted using MCF-7 cell line <em>in vitro.</em></p><p><strong>Methods: </strong>ACEi was purified by sequential steps of ethanol precipitation, ion exchange column chromatography (MonoQ) and gel filtration column chromatography (Sephadex G25). The apparent molecular mass was determined by SDS-PAGE. The anticancer property was analyzed by studying the cytotoxicity effects of angiotensin converting enzyme inhibitor using Breast cancer MCF-7 cell lines</p><p><strong>Results: </strong>The peptide was purified and molecular mass was determined as 4.5 kDa. The IC<sub>50</sub> value of peptide was found to be 59.5 µg/ml. The DNA fragmentation was not observed in the treated cells. The purified peptide has demonstrated to induce apoptosis of cancer cell. The results proved that the peptide has the ability to be used for cancer therapy.</p><p><strong>Conclusion: </strong>The presence of ACE inhibition activities in the fermentation of beef extract using <em>Micrococcus luteus</em> has been investigated. The Peptide has been determined as an active compound that inhibited the activity of ACE. These properties indicate the possibilities of<strong> </strong>the use of purified protein as a potent anticancer agent.<strong></strong></p><p><strong>Keywords: </strong>Angiotensin-converting enzyme inhibitors, <em>Micrococcus luteus</em>, Anti-proliferative, Anti-metastatic, MCF-7 cell line, Anticancer activity.</p> ER -